Chicken IL-1A (Interleukin 1 Alpha) ELISA Kit | IL-1A elisa kit

Chicken IL-1A (Interleukin 1 Alpha) ELISA Kit

Cat. Num. AAA7606966

• Gene Names: IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA
• Reactivity: Chicken
• Synonyms: IL-1A (Interleukin 1 Alpha); Chicken IL-1A (Interleukin 1 Alpha) ELISA Kit; IL-1A elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL-1alpha. No significant cross-reactivity or interference between IL-1alpha and analogues was observed.
• Sequence Length: 271

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 7.813-500pg/ml
• Sensitivity: 4.688pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-1A elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 27894330
• NCBI GeneID: 3552
• NCBI Accession #: NP_000566.3
• NCBI GenBank Nucleotide #: NM_000575.4
• UniProt Accession #: P01583; Q53QF9; Q7RU02
• Molecular Weight: 30,607 Da
• NCBI Official Full Name: interleukin-1 alpha
• NCBI Official Synonym Full Names: interleukin 1 alpha
• NCBI Official Symbol: IL1A
• NCBI Official Synonym Symbols: IL1; IL-1A; IL1F1; IL1-ALPHA
• NCBI Protein Information: interleukin-1 alpha
• UniProt Protein Name: Interleukin-1 alpha
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL1F1; IL-1 alpha

NCBI Description

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.