ABCC2 sirna

ABCC2 siRNA (Rat)

Cat. Num. AAA8223977

• Gene Names: Abcc2; Mrp2; cMRP; Cmoat
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ABCC2; ABCC2 siRNA (Rat); CMOAT; CMRP; MRP2; Canalicular multispecific organic anion transporter 1; ATP-binding cassette sub-family C member 2; Canalicular multidrug resistance protein; Multidrug resistance-associated protein 2; ABCC2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ABCC2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1541

• Applicable Applications for ABCC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ABCC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ABCC2 sirna

siRNA to inhibit ABCC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6978669
• NCBI GeneID: 25303
• NCBI Accession #: NP_036965.1
• NCBI GenBank Nucleotide #: NM_012833.2
• UniProt Accession #: Q63120; Q63145
• Molecular Weight: 173,384 Da
• NCBI Official Full Name: canalicular multispecific organic anion transporter 1
• NCBI Official Synonym Full Names: ATP-binding cassette, subfamily C (CFTR/MRP), member 2
• NCBI Official Symbol: Abcc2
• NCBI Official Synonym Symbols: Mrp2; cMRP; Cmoat
• NCBI Protein Information: canalicular multispecific organic anion transporter 1
• UniProt Protein Name: Canalicular multispecific organic anion transporter 1
• Protein Family: ABC transporter C family
• UniProt Gene Name: Abcc2
• UniProt Synonym Gene Names: Cmoat; Cmrp; Mrp2
• UniProt Entry Name: MRP2_RAT

NCBI Description

multispecific organic anion transporter; mutation may be responsible for conjugated hyperbilirubinemia in the TR- rat, which is a model for human Dubin-Johnson syndrome [RGD, Feb 2006]

Uniprot Description

ABCC2: Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter. Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver. Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, ABC family

Cellular Component: intercellular canaliculus; cell surface; membrane; integral to plasma membrane; apical plasma membrane

Molecular Function: protein domain specific binding; protein binding; ATPase activity, coupled to transmembrane movement of substances; organic anion transmembrane transporter activity; ATP binding

Biological Process: response to drug; prostaglandin transport; response to estrogen stimulus; response to heat; metabolic process; response to steroid hormone stimulus; response to arsenic; multidrug transport; response to methotrexate; response to oxidative stress; cellular chloride ion homeostasis; organic anion transport