IDOL Blocking Peptide | MYLIP blocking peptide

IDOL Blocking Peptide

Cat. Num. AAA8224299

• Gene Names: MYLIP; MIR; IDOL
• Reactivity: Human, Mouse, Bovine, Monkey
• Applications: Blocking (BL)
• Purity: >85%
• Synonyms: IDOL; IDOL Blocking Peptide; BZF1; E3 ubiquitin-protein ligase MYLIP; Inducible degrader of the LDL-receptor; Idol; Myosin regulatory light chain interacting protein; MIR; MYLIP blocking peptide

• Host: Synthetic
• Reactivity: Human, Mouse, Bovine, Monkey
• Purity/Purification: >85%
• Form/Format: Lyophilized powder
• Sequence Length: 445

• Applicable Applications for MYLIP blocking peptide: Blocking (BL)
• Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry.
• Directions for Use: Blocking Peptide to the diluted primary antibody in a molar ratio of 10:1 (peptide to antibody) and incubate the mixture at 4 degree C for overnight or at room temperature for 2 hours.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYLIP blocking peptide

The peptide is used to block Anti-IDOL Antibody reactivity.

NCBI and Uniprot Product Information

• NCBI GI #: 38788243
• NCBI GeneID: 29116
• NCBI Accession #: NP_037394.2
• NCBI GenBank Nucleotide #: NM_013262.3
• UniProt Accession #: Q8WY64; Q5TIA4; Q9BU73; Q9NRL9; Q9UHE7
• Molecular Weight: 14,989 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase MYLIP
• NCBI Official Synonym Full Names: myosin regulatory light chain interacting protein
• NCBI Official Symbol: MYLIP
• NCBI Official Synonym Symbols: MIR; IDOL
• NCBI Protein Information: E3 ubiquitin-protein ligase MYLIP
• UniProt Protein Name: E3 ubiquitin-protein ligase MYLIP
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: MYLIP
• UniProt Synonym Gene Names: BZF1; IDOL; Idol; MIR
• UniProt Entry Name: MYLIP_HUMAN

NCBI Description

The ERM protein family members ezrin, radixin, and moesin are cytoskeletal effector proteins linking actin to membrane-bound proteins at the cell surface. Myosin regulatory light chain interacting protein (MYLIP) is a novel ERM-like protein that interacts with myosin regulatory light chain and inhibits neurite outgrowth. [provided by RefSeq, Jul 2008]

Uniprot Description

MYLIP: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol- dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Motility/polarity/chemotaxis; Ubiquitin ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: extrinsic to membrane; cytoskeleton; cytoplasm; plasma membrane; intracellular

Molecular Function: protein binding; zinc ion binding; cytoskeletal protein binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: nervous system development; cholesterol homeostasis; protein destabilization; protein ubiquitination during ubiquitin-dependent protein catabolic process; positive regulation of protein catabolic process; protein ubiquitination; regulation of low-density lipoprotein receptor catabolic process; cell motility