WNT9B sirna

WNT9B siRNA (Mouse)

Cat. Num. AAA8223912

• Gene Names: Wnt9b; clf; clf1; Wnt15; Wnt14b; wnt-15; wnt-14b
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WNT9B; WNT9B siRNA (Mouse); WNT14B; WNT15; Protein Wnt-9b; Protein Wnt-14b; Protein Wnt-15; WNT9B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WNT9B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 359

• Applicable Applications for WNT9B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WNT9B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WNT9B sirna

siRNA to inhibit WNT9B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 238231388
• NCBI GeneID: 22412
• NCBI Accession #: NP_035849.3
• NCBI GenBank Nucleotide #: NM_011719.4
• UniProt Accession #: O35468; Q8VI90
• Molecular Weight: 38,982 Da
• NCBI Official Full Name: protein Wnt-9b
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 9B
• NCBI Official Symbol: Wnt9b
• NCBI Official Synonym Symbols: clf; clf1; Wnt15; Wnt14b; wnt-15; wnt-14b
• NCBI Protein Information: protein Wnt-9b
• UniProt Protein Name: Protein Wnt-9b
• Protein Family: Protein
• UniProt Gene Name: Wnt9b
• UniProt Synonym Gene Names: Wnt14b; Wnt15
• UniProt Entry Name: WNT9B_MOUSE

Uniprot Description

WNT9B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region

Molecular Function: frizzled binding; receptor binding

Biological Process: positive regulation of catalytic activity; regulation of protein amino acid phosphorylation; Wnt receptor signaling pathway; cell fate commitment; regulation of asymmetric cell division; in utero embryonic development; Wnt receptor signaling pathway, planar cell polarity pathway; multicellular organismal development; embryonic cranial skeleton morphogenesis; Wnt receptor signaling pathway through beta-catenin; palate development; cellular response to starvation; signal transduction; neuron differentiation; organ morphogenesis; cell-cell signaling; branching morphogenesis of a tube; ureteric bud branching; male genitalia development; kidney development; regulation of tube size