WBSCR17 sirna

WBSCR17 siRNA (Mouse)

Cat. Num. AAA8211609

• Gene Names: Wbscr17; AA388321; E330012B09Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WBSCR17; WBSCR17 siRNA (Mouse); GALNTL3; Putative polypeptide N-acetylgalactosaminyltransferase-like protein 3; Polypeptide GalNAc transferase-like protein 3; GalNAc-T-like protein 3; pp-GaNTase-like protein 3; Protein-UDP acetylgalactosaminyltransferase-like protein 3; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 3; Williams-Beuren syndrome chromosomal region 17 protein homolog; WBSCR17 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WBSCR17 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 598

• Applicable Applications for WBSCR17 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WBSCR17 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WBSCR17 sirna

siRNA to inhibit WBSCR17 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 32401429
• NCBI GeneID: 212996
• NCBI Accession #: NP_660253.2
• NCBI GenBank Nucleotide #: NM_145218.3
• UniProt Accession #: Q7TT15; Q8BKN7; Q8BUY1; Q8BX73; Q8BZC8; Q8K483
• Molecular Weight: 67,516 Da
• NCBI Official Full Name: putative polypeptide N-acetylgalactosaminyltransferase-like protein 3
• NCBI Official Synonym Full Names: Williams-Beuren syndrome chromosome region 17 homolog (human)
• NCBI Official Symbol: Wbscr17
• NCBI Official Synonym Symbols: AA388321; E330012B09Rik
• NCBI Protein Information: putative polypeptide N-acetylgalactosaminyltransferase-like protein 3
• UniProt Protein Name: Putative polypeptide N-acetylgalactosaminyltransferase-like protein 3
• Protein Family: Putative polypeptide N-acetylgalactosaminyltransferase-like protein
• UniProt Gene Name: Wbscr17
• UniProt Synonym Gene Names: Galntl3; GalNAc-T-like protein 3; pp-GaNTase-like protein 3
• UniProt Entry Name: GLTL3_MOUSE

Uniprot Description

WBSCR17: May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D- galactosamine residue to a serine or threonine residue on the protein receptor. WBSCR17 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.

Protein type: Glycan Metabolism - O-glycan biosynthesis; EC 2.4.1.41; Transferase; Membrane protein, integral

Cellular Component: Golgi apparatus; membrane; integral to membrane

Molecular Function: alpha(1,6)-fucosyltransferase activity; acetylgalactosaminyltransferase activity; metal ion binding; oligosaccharyl transferase activity; alpha(1,3)-fucosyltransferase activity; glucosyltransferase activity; O antigen polymerase activity; alpha-1,2-mannosyltransferase activity; acetylglucosaminyltransferase activity; transferase activity, transferring glycosyl groups; 1-phenanthrol glycosyltransferase activity; phenanthrol glycosyltransferase activity; polypeptide N-acetylgalactosaminyltransferase activity; glycolipid mannosyltransferase activity; carbohydrate binding; galactosyltransferase activity; dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase activity; dolichyl pyrophosphate Man7GlcNAc2 alpha-1,3-glucosyltransferase activity; fucosyltransferase activity; lipopolysaccharide-1,6-galactosyltransferase activity; alpha-1,2-galactosyltransferase activity; alpha-1,3-galactosyltransferase activity; 9-phenanthrol UDP-glucuronosyltransferase activity; dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; UDP-glucose:glycoprotein glucosyltransferase activity; 1,2-dihydroxy-phenanthrene glycosyltransferase activity; 9-phenanthrol glycosyltransferase activity; transferase activity; dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity; cellulose synthase activity; beta-1,4-mannosyltransferase activity; mannosyltransferase activity; alpha-1,3-mannosyltransferase activity; alpha-1,6-mannosyltransferase activity; inositol phosphoceramide synthase activity