Human Lysyl Oxidase Like Protein 3 (LOXL3) RTU ELISA Kit | LOXL3 rtu elisa kit

Human Lysyl Oxidase Like Protein 3 (LOXL3) ELISA Kit

Cat. Num. AAA4500814

• Gene Names: LOXL3; LOXL
• Reactivity: Human
• Synonyms: Lysyl Oxidase Like Protein 3 (LOXL3); Human Lysyl Oxidase Like Protein 3 (LOXL3) ELISA Kit; LOXL; Lysyl oxidase homolog 3; LOXL3 rtu elisa kit

• Reactivity: Human
• Sequence Length: 608

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.072ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LOXL3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LOXL3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LOXL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LOXL3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of LOXL3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 574957232
• NCBI GeneID: 84695
• NCBI Accession #: NP_001276093.1
• NCBI GenBank Nucleotide #: NM_001289164.2
• UniProt Accession #: P58215; Q2EHP2; Q6IPL7; Q96RS1; D6W5J1
• Molecular Weight: 67,445 Da
• NCBI Official Full Name: lysyl oxidase homolog 3 isoform 2
• NCBI Official Synonym Full Names: lysyl oxidase like 3
• NCBI Official Symbol: LOXL3
• NCBI Official Synonym Symbols: LOXL
• NCBI Protein Information: lysyl oxidase homolog 3
• UniProt Protein Name: Lysyl oxidase homolog 3
• Protein Family: Lysyl oxidase
• UniProt Gene Name: LOXL3

NCBI Description

This gene encodes a lysyl oxidase, which likely functions as an amine oxidase and plays a role in the formation of crosslinks in collagens and elastin. Deletion of the related gene in mouse causes neonatal mortality with cleft palate, spine deformity, and defects in collagen organization. A mutation in this gene was found in a family with Stickler syndrome. [provided by RefSeq, Sep 2016]

Uniprot Description

Protein-lysine 6-oxidase that mediates the oxidation of peptidyl lysine residues to allysine in target proteins (PubMed:17018530, PubMed:28065600). Catalyzes the post-translational oxidative deamination of peptidyl lysine residues in precursors of elastin and different types of collagens, a prerequisite in the formation of cross-links between collagens and elastin (PubMed:17018530). Required for somite boundary formation by catalyzing oxidation of fibronectin (FN1), enhancing integrin signaling in myofibers and their adhesion to the myotendinous junction (MTJ) (). Acts as a regulator of inflammatory response by inhibiting differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells (Treg): acts by interacting with STAT3 in the nucleus and catalyzing both deacetylation and oxidation of lysine residues on STAT3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600). Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated (PubMed:28065600). Also able to catalyze deacetylation of lysine residues on STAT3 (PubMed:28065600).