VPS28 sirna

VPS28 siRNA (Mouse)

Cat. Num. AAA8204414

• Gene Names: Vps28; CIIA; 1110014J03Rik; D730005C08Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VPS28; VPS28 siRNA (Mouse); Vacuolar protein sorting-associated protein 28 homolog; Caspase-activated DNase inhibitor that interacts with ASK1; CIIA; ESCRT-I complex subunit VPS28; VPS28 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: VPS28 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 183

• Applicable Applications for VPS28 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse VPS28 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VPS28 sirna

siRNA to inhibit VPS28 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 787168010
• NCBI GeneID: 66914
• NCBI Accession #: NP_001292597.1
• NCBI GenBank Nucleotide #: NM_001305668.1
• UniProt Accession #: Q9D1C8
• Molecular Weight: 25,452 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 28 homolog isoform 2
• NCBI Official Synonym Full Names: vacuolar protein sorting 28 (yeast)
• NCBI Official Symbol: Vps28
• NCBI Official Synonym Symbols: CIIA; 1110014J03Rik; D730005C08Rik
• NCBI Protein Information: vacuolar protein sorting-associated protein 28 homolog
• UniProt Protein Name: Vacuolar protein sorting-associated protein 28 homolog
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: Vps28
• UniProt Synonym Gene Names: CIIA
• UniProt Entry Name: VPS28_MOUSE

NCBI Description

This gene encodes a protein which is thought to be a subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]

Uniprot Description

VPS28: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Belongs to the VPS28 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Cellular Component: cytoplasm; endosome membrane; cytosol; endosome

Molecular Function: protein binding; protein complex binding

Biological Process: ubiquitin-dependent protein catabolic process via the multivesicular body pathway; negative regulation of protein ubiquitination; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway