Human BTG3 Associated Nuclear Protein (BANP) ELISA Kit | BANP elisa kit

Human BTG3 Associated Nuclear Protein (BANP) ELISA Kit

Cat. Num. AAA453490

• Gene Names: BANP; BEND1; SMAR1; SMARBP1
• Reactivity: Human
• Synonyms: BTG3 Associated Nuclear Protein (BANP); Human BTG3 Associated Nuclear Protein (BANP) ELISA Kit; SMAR1; SMARBP1; BEND1; BEN Domain Containing 1; Scaffold/matrix-associated region-1-binding protein; BANP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of BANP. No significant cross-reactivity or interference between BANP and analogues was observed.
• Sequence Length: 505

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.055ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BANP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BANP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BANP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BANP in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BANP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to BANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BANP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 291084791
• NCBI GeneID: 54971
• NCBI Accession #: NP_001167010.1
• NCBI GenBank Nucleotide #: NM_001173539.1
• UniProt Accession #: Q8N9N5; Q96GJ7; Q9NWY1; A8MU25; A8MX25; B2RCF7; B4DNJ9; F5GZM0
• Molecular Weight: 55,502 Da
• NCBI Official Full Name: protein BANP isoform c
• NCBI Official Synonym Full Names: BTG3 associated nuclear protein
• NCBI Official Symbol: BANP
• NCBI Official Synonym Symbols: BEND1; SMAR1; SMARBP1
• NCBI Protein Information: protein BANP
• UniProt Protein Name: Protein BANP
• Protein Family: Protein
• UniProt Gene Name: BANP
• UniProt Synonym Gene Names: BEND1; SMAR1

NCBI Description

This gene encodes a protein that binds to matrix attachment regions. The protein forms a complex with p53 and negatively regulates p53 transcription, and functions as a tumor suppressor and cell cycle regulator. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

Controls V(D)J recombination during T-cell development by repressing T-cell receptor (TCR) beta enhancer function. Binds to scaffold/matrix attachment region beta (S/MARbeta), an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Represses cyclin D1 transcription by recruiting HDAC1 to its promoter, thereby diminishing H3K9ac, H3S10ph and H4K8ac levels. Promotes TP53 'Ser-15' phosphorylation and nuclear accumulation, which causes cell cycle arrest ().