VAV3 sirna

VAV3 siRNA (Mouse)

Cat. Num. AAA8217904

• Gene Names: Vav3; Idd18.1; AA986410; A530094I06Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VAV3; VAV3 siRNA (Mouse); Guanine nucleotide exchange factor VAV3; VAV-3; VAV3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: VAV3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 574

• Applicable Applications for VAV3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse VAV3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VAV3 sirna

siRNA to inhibit VAV3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 69724858
• NCBI GeneID: 57257
• NCBI Accession #: NP_065251.2
• NCBI GenBank Nucleotide #: NM_020505.2
• UniProt Accession #: Q9R0C8; Q7TS85; Q8BRV2; Q8CCF5; Q8R076; Q9JLS6; A2CFD7
• Molecular Weight: 66,849 Da
• NCBI Official Full Name: guanine nucleotide exchange factor VAV3 isoform 1
• NCBI Official Synonym Full Names: vav 3 oncogene
• NCBI Official Symbol: Vav3
• NCBI Official Synonym Symbols: Idd18.1; AA986410; A530094I06Rik
• NCBI Protein Information: guanine nucleotide exchange factor VAV3
• UniProt Protein Name: Guanine nucleotide exchange factor VAV3
• Protein Family: Guanine nucleotide exchange factor
• UniProt Gene Name: Vav3
• UniProt Synonym Gene Names: VAV-3
• UniProt Entry Name: VAV3_MOUSE

Uniprot Description

VAV3: a guanine nucleotide exchange factor (GEF) that activates RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by activated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion. Interacts with the PH domain of APS. Interacts (via SH2 domains) with the phosphorylated form of EPHA2. Interacts with ROS1; constitutive interaction that mediates VAV3 phosphorylation. Down-regulated by EGF and TGF-beta. Four isoforms of the human protein are produced by alternative promoter. Isoform 1 and isoform 3 are widely expressed; both are expressed at very low levels in skeletal muscle. In keratinocytes, isoform 1 is less abundant than isoform 3. Isoform 3 is detected at very low levels, if any, in adrenal gland, bone marrow, spleen, fetal brain and spinal chord; in these tissues, isoform 1 is readily detectable.

Protein type: Actin-binding; Nuclear receptor co-regulator; GEFs, Rac/Rho; Motility/polarity/chemotaxis; GEFs; Adaptor/scaffold

Cellular Component: cytoplasm; plasma membrane; intracellular

Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; Rac guanyl-nucleotide exchange factor activity; metal ion binding; guanyl-nucleotide exchange factor activity; epidermal growth factor receptor binding

Biological Process: lamellipodium biogenesis; response to drug; integrin-mediated signaling pathway; neutrophil chemotaxis; regulation of GTPase activity; cell migration; positive regulation of cell adhesion; positive regulation of phosphoinositide 3-kinase activity; cell projection biogenesis; vesicle fusion; B cell receptor signaling pathway; small GTPase mediated signal transduction; regulation of Rho protein signal transduction; positive regulation of B cell proliferation; angiogenesis; response to DNA damage stimulus; positive regulation of GTPase activity