UST sirna

UST siRNA (Mouse)

Cat. Num. AAA8209314

• Gene Names: Ust; UA2OST; D930010O20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UST; UST siRNA (Mouse); Uronyl 2-sulfotransferase; UST sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: UST siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 407

• Applicable Applications for UST sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse UST gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UST sirna

siRNA to inhibit UST expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110556631
• NCBI GeneID: 338362
• NCBI Accession #: NP_796361.2
• NCBI GenBank Nucleotide #: NM_177387.3
• UniProt Accession #: Q8BUB6; B2RR02
• Molecular Weight: 47,730 Da
• NCBI Official Full Name: uronyl 2-sulfotransferase
• NCBI Official Synonym Full Names: uronyl-2-sulfotransferase
• NCBI Official Symbol: Ust
• NCBI Official Synonym Symbols: UA2OST; D930010O20Rik
• NCBI Protein Information: uronyl 2-sulfotransferase
• UniProt Protein Name: Uronyl 2-sulfotransferase
• Protein Family: Uronyl 2-sulfotransferase
• UniProt Gene Name: Ust
• UniProt Entry Name: UST_MOUSE

Uniprot Description

UST: Sulfotransferase that catalyzes the transfer of sulfate to the position 2 of uronyl residues. Has mainly activity toward iduronyl residues in dermatan sulfate, and weaker activity toward glucuronyl residues of chondroitin sulfate. Has no activity toward desulfated N-resulfated heparin. Belongs to the sulfotransferase 3 family.

Protein type: Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral; EC 2.8.2.-; Transferase

Cellular Component: Golgi apparatus; membrane; integral to membrane

Molecular Function: phenanthrol sulfotransferase activity; proteoglycan sulfotransferase activity; trans-3,4-dihydrodiolphenanthrene sulfotransferase activity; 2-phenanthrol sulfotransferase activity; galactose 3-O-sulfotransferase activity; transferase activity; 1-phenanthrol sulfotransferase activity; 3-phenanthrol sulfotransferase activity; sulfotransferase activity; HNK-1 sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity; 4-phenanthrol sulfotransferase activity; cholesterol sulfotransferase activity; heparan sulfate 2-O-sulfotransferase activity; trans-9R,10R-dihydrodiolphenanthrene sulfotransferase activity; N-acetylgalactosamine 4-O-sulfotransferase activity; 9-phenanthrol sulfotransferase activity; heparan sulfate 6-O-sulfotransferase activity