Mouse Ephrin type-B receptor 4 ELISA Kit | Ephb4 elisa kit

Mouse Ephrin type-B receptor 4 ELISA Kit

Cat. Num. AAA761505

• Gene Names: EPHB4; HTK; MYK1; TYRO11
• Reactivity: Mouse
• Synonyms: Ephrin type-B receptor 4; Mouse Ephrin type-B receptor 4 ELISA Kit; Ephb4/Tyrosine-protein kinase TYRO11/Tyrosine-protein kinase receptor HTK/EPHB4/HTK/TYRO11/2.7.10.1/TYRO11; Ephb4 elisa kit

• Reactivity: Mouse
• Sequence Length: 987

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Ephb4 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Ephb4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Ephb4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Ephb4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Ephb4 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 32528301
• NCBI GeneID: 2050
• NCBI Accession #: NP_004435.3
• NCBI GenBank Nucleotide #: NM_004444.4
• Molecular Weight: 45,195 Da
• NCBI Official Full Name: ephrin type-B receptor 4
• NCBI Official Synonym Full Names: EPH receptor B4
• NCBI Official Symbol: EPHB4
• NCBI Official Synonym Symbols: HTK; MYK1; TYRO11
• NCBI Protein Information: ephrin type-B receptor 4
• UniProt Protein Name: Ephrin type-B receptor 4
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: EPHB4
• UniProt Synonym Gene Names: HTK; MYK1; TYRO11
• UniProt Entry Name: EPHB4_HUMAN

NCBI Description

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq, Jul 2008]

Uniprot Description

EphB4: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4- mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Protein type: Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; TK group; Eph family

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: cytosol; extracellular region; integral to plasma membrane; plasma membrane

Molecular Function: ATP binding; ephrin receptor activity; protein binding; transmembrane receptor protein tyrosine kinase activity

Biological Process: angiogenesis; axon guidance; cell adhesion; cell migration during sprouting angiogenesis; ephrin receptor signaling pathway; heart morphogenesis; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation