USP13 sirna

USP13 siRNA (Mouse)

Cat. Num. AAA8211451

• Gene Names: Usp13; ISOT3; IsoT-3; AI848077; 2700071E21Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: USP13; USP13 siRNA (Mouse); Ubiquitin carboxyl-terminal hydrolase 13; Deubiquitinating enzyme 13; Ubiquitin thioesterase 13; Ubiquitin-specific-processing protease 13; USP13 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: USP13 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 858

• Applicable Applications for USP13 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse USP13 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for USP13 sirna

siRNA to inhibit USP13 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 61316410
• NCBI GeneID: 72607
• NCBI Accession #: NP_001013042.1
• NCBI GenBank Nucleotide #: NM_001013024.2
• UniProt Accession #: Q5BKP2; D3YYG7
• Molecular Weight: 96,723 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 13
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 13 (isopeptidase T-3)
• NCBI Official Symbol: Usp13
• NCBI Official Synonym Symbols: ISOT3; IsoT-3; AI848077; 2700071E21Rik
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 13
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 13
• UniProt Gene Name: Usp13
• UniProt Entry Name: UBP13_MOUSE

Uniprot Description

USP13: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. Belongs to the peptidase C19 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.19.12; Ubiquitin conjugating system; Protease; Ubiquitin-specific protease

Cellular Component: lysosome

Molecular Function: peptidase activity; omega peptidase activity; ubiquitin binding; hydrolase activity; zinc ion binding; metal ion binding; cysteine-type endopeptidase activity; ubiquitin protein ligase binding; ubiquitin-specific protease activity; cysteine-type peptidase activity

Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; protein deubiquitination; protein stabilization; regulation of transcription, DNA-dependent; autophagy; regulation of autophagy; proteolysis