USH1C sirna

USH1C siRNA (Mouse)

Cat. Num. AAA8239015

• Gene Names: Ush1c; harmonin; 2010016F01Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: USH1C; USH1C siRNA (Mouse); Harmonin; PDZ domain-containing protein; Usher syndrome type-1C protein homolog; USH1C sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: USH1C siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 891

• Applicable Applications for USH1C sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse USH1C gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for USH1C sirna

siRNA to inhibit USH1C expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 254911005
• NCBI GeneID: 72088
• NCBI Accession #: NP_001157205.1
• NCBI GenBank Nucleotide #: NM_001163733.1
• UniProt Accession #: Q9ES64; Q91XD1; Q9CVG7; Q9ES65
• Molecular Weight: 68,185 Da
• NCBI Official Full Name: harmonin isoform b4
• NCBI Official Synonym Full Names: Usher syndrome 1C
• NCBI Official Symbol: Ush1c
• NCBI Official Synonym Symbols: harmonin; 2010016F01Rik
• NCBI Protein Information: harmonin
• UniProt Protein Name: Harmonin
• Protein Family: Harmonin
• UniProt Gene Name: Ush1c
• UniProt Entry Name: USH1C_MOUSE

Uniprot Description

USH1C: Required for normal development and maintenance of cochlear hair cell bundles. Anchoring/scaffolding protein that is a part of the functional network formed by USH1C, USH1G, CDH23 and MYO7A that mediates mechanotransduction in cochlear hair cells. Required for normal hearing. Defects in USH1C are the cause of Usher syndrome type 1C (USH1C); also known as Usher syndrome type I Acadian variety. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH1 is characterized by profound congenital sensorineural deafness, absent vestibular function and prepubertal onset of progressive retinitis pigmentosa leading to blindness. Defects in USH1C are the cause of deafness autosomal recessive type 18 (DFNB18). DFNB18 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Cellular Component: stereocilium; cytoskeleton; photoreceptor inner segment; photoreceptor outer segment; apical part of cell; cytoplasm; plasma membrane; synapse; brush border

Molecular Function: actin filament binding; protein binding; myosin tail binding; spectrin binding

Biological Process: actin filament bundle formation; parallel actin filament bundle formation; inner ear morphogenesis; sensory perception of sound; sensory perception of light stimulus; photoreceptor cell maintenance; auditory receptor cell differentiation; neuromuscular process controlling balance; G2/M transition of mitotic cell cycle; equilibrioception