UMPS sirna

UMPS siRNA (Mouse)

Cat. Num. AAA8220654

• Gene Names: Umps; AA408257; AL033308; BB164745; 1700095D23Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UMPS; UMPS siRNA (Mouse); Uridine 5'-monophosphate synthase; UMP synthase; UMPS sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: UMPS siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 481

• Applicable Applications for UMPS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse UMPS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UMPS sirna

siRNA to inhibit UMPS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 33859498
• NCBI GeneID: 22247
• NCBI Accession #: NP_033497.1
• NCBI GenBank Nucleotide #: NM_009471.2
• UniProt Accession #: P13439; Q99L26
• Molecular Weight: 52,292 Da
• NCBI Official Full Name: uridine 5'-monophosphate synthase
• NCBI Official Synonym Full Names: uridine monophosphate synthetase
• NCBI Official Symbol: Umps
• NCBI Official Synonym Symbols: AA408257; AL033308; BB164745; 1700095D23Rik
• NCBI Protein Information: uridine 5'-monophosphate synthase
• UniProt Protein Name: Uridine 5'-monophosphate synthase
• Protein Family: Uridine 5'-monophosphate synthase
• UniProt Gene Name: Umps
• UniProt Synonym Gene Names: UMP synthase; OPRTase
• UniProt Entry Name: UMPS_MOUSE

Uniprot Description

UMPS: Defects in UMPS are the cause of orotic aciduria type 1 (ORAC1). A disorder of pyrimidine metabolism resulting in megaloblastic anemia and orotic acid crystalluria that is frequently associated with some degree of physical and mental retardation. A minority of cases have additional features, particularly congenital malformations and immune deficiencies. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.4.2.10; Nucleotide Metabolism - pyrimidine; Xenobiotic Metabolism - drug metabolism - other enzymes; Lyase; EC 4.1.1.23

Cellular Component: cytoplasm; nucleus

Molecular Function: orotate phosphoribosyltransferase activity; transferase activity; transferase activity, transferring glycosyl groups; orotidine-5'-phosphate decarboxylase activity; lyase activity; carboxy-lyase activity; catalytic activity

Biological Process: 'de novo' pyrimidine base biosynthetic process; metabolic process; UMP biosynthetic process; nucleoside metabolic process; pyrimidine nucleotide biosynthetic process