UCHL1 sirna

UCHL1 siRNA (Mouse)

Cat. Num. AAA8234989

• Gene Names: Uchl1; gad; C88048; PGP9.5; R75593; UCH-L1; UCHL-1; PGP 9.5; AW822034
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UCHL1; UCHL1 siRNA (Mouse); Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: UCHL1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 223

• Applicable Applications for UCHL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse UCHL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UCHL1 sirna

siRNA to inhibit UCHL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 188219614
• NCBI GeneID: 22223
• NCBI Accession #: NP_035800.2
• NCBI GenBank Nucleotide #: NM_011670.2
• UniProt Accession #: Q9R0P9; Q9R122
• Molecular Weight: 24,838 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin carboxy-terminal hydrolase L1
• NCBI Official Symbol: Uchl1
• NCBI Official Synonym Symbols: gad; C88048; PGP9.5; R75593; UCH-L1; UCHL-1; PGP 9.5; AW822034
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: Uchl1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_MOUSE

Uniprot Description

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: Ligase; EC 3.4.19.12; Cell development/differentiation; Protease; Ubiquitin conjugating system

Cellular Component: nucleoplasm; membrane; cell soma; endoplasmic reticulum; axon; cell; cytoplasm; plasma membrane; intracellular; cytosol; myelin sheath

Molecular Function: peptidase activity; omega peptidase activity; ubiquitin binding; hydrolase activity; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity; cysteine-type peptidase activity

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of MAP kinase activity; protein deubiquitination; eating behavior; neuromuscular process; proteolysis; sensory perception of pain; adult walking behavior; cell proliferation; axon target recognition; axon transport of mitochondrion; axonogenesis; muscle fiber development