Western Blot (WB)
(Anti-Myelin Basic Protein antibody, MBS175140, Western blottingWB: Mouse Brain Tissue Lysate )
Rabbit Myelin Basic Protein Polyclonal Antibody | anti-MBP antibody
Anti-Myelin Basic Protein antibody
IHC (Paraffin-embedded Section): Concentration: 0.5-1ug/ml; Tested Species: Ms, Rat; Predicted Species: Hu; Antigen Retrieval: By Heat
IF: Concentration: 2ug/ml; Tested Species: Ms, Rat; Predicted Species: Hu
Tested Species: In house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similiarities.
By Heat: Boiling paraffin sections in 10mM citrate buffer pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supporter by chemiluminescence kit MBS176460 in WB, supported by MBS176451 in IHC(P)
Western Blot (WB)
(Anti-Myelin Basic Protein antibody, MBS175140, Western blottingWB: Mouse Brain Tissue Lysate )
Western Blot (WB)
(Anti-Myelin Basic Protein antibody, MBS175140, Western blottingWB: Mouse Brain Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-Myelin Basic Protein antibody, MBS175140, IHC(P)IHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-Myelin Basic Protein antibody, MBS175140, IHC(P)IHC(P): Rat Brain Tissue )
Background: Myelin basic protein(MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008]
Uniprot Description
MBP: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation. Homodimer. Isoform 3 exists as a homodimer. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. Belongs to the myelin basic protein family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 18q23
Cellular Component: compact myelin; internode region of axon; cell soma; plasma membrane; nucleus
Molecular Function: protein binding; protease binding; structural constituent of myelin sheath
Biological Process: myelination; response to mercury ion; synaptic transmission; substantia nigra development; central nervous system development; response to toxin; negative regulation of axonogenesis; immune response; axon ensheathment; response to progesterone stimulus; aging
Research Articles on MBP
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Product Notes
The MBP mbp (Catalog #AAA175140) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Myelin Basic Protein antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Myelin Basic Protein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluoresence (IF). WB: Concentration 0.1-0.5ug/ml; Tested Species: Hu, Ms, Rat IHC (Paraffin-embedded Section): Concentration: 0.5-1ug/ml; Tested Species: Ms, Rat; Predicted Species: Hu; Antigen Retrieval: By Heat IF: Concentration: 2ug/ml; Tested Species: Ms, Rat; Predicted Species: Hu Tested Species: In house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similiarities. By Heat: Boiling paraffin sections in 10mM citrate buffer pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Antibody can be supporter by chemiluminescence kit MBS176460 in WB, supported by MBS176451 in IHC(P). Researchers should empirically determine the suitability of the MBP mbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myelin Basic Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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