UBR5 sirna

UBR5 siRNA (Mouse)

Cat. Num. AAA8226934

• Gene Names: Ubr5; Edd; Edd1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: UBR5; UBR5 siRNA (Mouse); EDD; EDD1; KIAA0896; E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase. HECT domain-containing 1; Hyperplastic discs protein homolog; UBR5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: UBR5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2,792

• Applicable Applications for UBR5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse UBR5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for UBR5 sirna

siRNA to inhibit UBR5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 163310751
• NCBI GeneID: 70790
• NCBI Accession #: NP_001074828.2
• NCBI GenBank Nucleotide #: NM_001081359.3
• UniProt Accession #: Q80TP3; Q698K9; Q6PEQ8; Q6PFQ9; Q80VL4; Q810V6; Q9CXE9
• Molecular Weight: 308,352 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase UBR5 isoform 1
• NCBI Official Synonym Full Names: ubiquitin protein ligase E3 component n-recognin 5
• NCBI Official Symbol: Ubr5
• NCBI Official Synonym Symbols: Edd; Edd1
• NCBI Protein Information: E3 ubiquitin-protein ligase UBR5
• UniProt Protein Name: E3 ubiquitin-protein ligase UBR5
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Ubr5
• UniProt Synonym Gene Names: Edd; Edd1; Kiaa0896
• UniProt Entry Name: UBR5_MOUSE

Uniprot Description

UBR5: E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Binds TOPBP1. Associates with CDK9 and TFIIS/TCEA1 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulates target gene transcription (e.g. gamma fibrinogen/FGG) by recruiting their promoters. Associates with the E3 ligase complex containing DYRK2, EDD/UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts directly with DYRK2. Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.

Protein type: Ligase; Ubiquitin conjugating system; Ubiquitin ligase; EC 6.3.2.-; EC 6.3.2.19; Nuclear receptor co-regulator

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: ubiquitin binding; zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; RNA binding; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: protein polyubiquitination; progesterone receptor signaling pathway; positive regulation of protein import into nucleus, translocation; protein ubiquitination during ubiquitin-dependent protein catabolic process; DNA repair; response to DNA damage stimulus