Testing Data
(Lymphocytes gated PBMCs stained with 367100 (clone OKT1, red histogram). PBMCs lymphocytes stained with IgG1 APC isotype control (blue histogram).)
Mouse anti-Human Oncofetal Antigen 5T4 Monoclonal Antibody
Oncofetal Antigen 5T4 (TPBG, Trophoblast Glycoprotein, 5T4 Oncofetal antigen, 5T4 Oncofetal Trophoblast Glycoprotein, M6P1)
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Oncofetal Antigen 5T4; Monoclonal Antibody; Oncofetal Antigen 5T4 (TPBG; Trophoblast Glycoprotein; 5T4 Oncofetal antigen; 5T4 Oncofetal Trophoblast Glycoprotein; M6P1); Anti -Oncofetal Antigen 5T4 (TPBG; anti-Oncofetal Antigen 5T4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9i235
Specificity
Recognizes human Oncofetal Antigen 5T4. Does not cross-react with mouse.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Applicable Applications for anti-Oncofetal Antigen 5T4 antibody
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Direct ELISA and Flow Cytometry.
Dilution: Flow Cytometry: 2.5ug/10e6 cells. Tested with BG01V human embryonic stem cells.
Dilution: Flow Cytometry: 2.5ug/10e6 cells. Tested with BG01V human embryonic stem cells.
Immunogen
Recombinant protein corresponding to aa31-355 of human Oncofetal Antigen 5T4 expressed in NSO cells. (Q13641)
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Lymphocytes gated PBMCs stained with 367100 (clone OKT1, red histogram). PBMCs lymphocytes stained with IgG1 APC isotype control (blue histogram).)
Testing Data
(Lymphocytes gated PBMCs stained with 367100 (clone OKT1, red histogram). PBMCs lymphocytes stained with IgG1 APC isotype control (blue histogram).)
Related Product Information for anti-Oncofetal Antigen 5T4 antibody
Oncofetal antigen 5T4, also TPBG and trophoblast glycoprotein, is a 72kD glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and embryonic stem cells, and impacts cell adhesion and motility. The human 5T4 cDNA encodes a type I transmembrane protein precursor that is 420 amino acids in length. It contains a 324aa extracellular region (aa 32-355) that shows one Ser-rich region followed by seven Leu-rich repeats (aa90-355). Over aa31-355, human 5T4 shares 81% and 85% aa identity with mouse and canine 5T4, respectively.
Product Categories/Family for anti-Oncofetal Antigen 5T4 antibody
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Product Notes
The Oncofetal Antigen 5T4 (Catalog #AAA606018) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Oncofetal Antigen 5T4 (TPBG, Trophoblast Glycoprotein, 5T4 Oncofetal antigen, 5T4 Oncofetal Trophoblast Glycoprotein, M6P1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Oncofetal Antigen 5T4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Flow Cytometry (FC/FACS). Suitable for use in Direct ELISA and Flow Cytometry. Dilution: Flow Cytometry: 2.5ug/10e6 cells. Tested with BG01V human embryonic stem cells. Researchers should empirically determine the suitability of the Oncofetal Antigen 5T4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Oncofetal Antigen 5T4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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