TYSND1 sirna

TYSND1 siRNA (Mouse)

Cat. Num. AAA829158

• Gene Names: Tysnd1; 1300019N10Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TYSND1; TYSND1 siRNA (Mouse); Peroxisomal leader peptide-processing protease; Trypsin domain-containing protein 1; TYSND1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TYSND1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 521

• Applicable Applications for TYSND1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TYSND1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TYSND1 sirna

siRNA to inhibit TYSND1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 441418800
• NCBI GeneID: 71767
• NCBI Accession #: NP_001259019.1
• NCBI GenBank Nucleotide #: NM_001272090.1
• UniProt Accession #: Q9DBA6; Q0VE90
• Molecular Weight: 59,066 Da
• NCBI Official Full Name: peroxisomal leader peptide-processing protease isoform b
• NCBI Official Synonym Full Names: trypsin domain containing 1
• NCBI Official Symbol: Tysnd1
• NCBI Official Synonym Symbols: 1300019N10Rik
• NCBI Protein Information: peroxisomal leader peptide-processing protease
• UniProt Protein Name: Peroxisomal leader peptide-processing protease
• Protein Family: Peroxisomal leader peptide-processing protease
• UniProt Gene Name: Tysnd1
• UniProt Entry Name: TYSD1_MOUSE

NCBI Description

This gene encodes a protease that removes the N-terminal peroxisomal targeting signal (PTS2) from proteins produced in the cytosol, thereby facilitating their import into the peroxisome. The encoded protein is also capable of removing the C-terminal peroxisomal targeting signal (PTS1) from proteins in the peroxisomal matrix. The full-length protein undergoes self-cleavage to produce shorter, potentially inactive, peptides. Alternative splicing results in multiple transcript variants for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

TYSND1: Peroxisomal protease that mediates both the removal of the leader peptide from proteins containing a PTS2 target sequence and processes several PTS1-containing proteins. Catalyzes the processing of PTS1-proteins involved in the peroxisomal beta- oxidation of fatty acids. Belongs to the peptidase S1B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Protease

Cellular Component: membrane; peroxisome

Molecular Function: peptidase activity; identical protein binding; protease binding; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: regulation of fatty acid beta-oxidation; protein processing; proteolysis; protein homooligomerization