TOPBP1 sirna

TOPBP1 siRNA (Mouse)

Cat. Num. AAA8203205

• Gene Names: Topbp1; AI256758; mKIAA0259; 1110031N14Rik; 2810429C13Rik; D430026L04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TOPBP1; TOPBP1 siRNA (Mouse); KIAA0259; DNA topoisomerase 2-binding protein 1; DNA topoisomerase II-beta-binding protein 1; TopBP1; DNA topoisomerase II-binding protein 1; TOPBP1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TOPBP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1515

• Applicable Applications for TOPBP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TOPBP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TOPBP1 sirna

siRNA to inhibit TOPBP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31559817
• NCBI GeneID: 235559
• NCBI Accession #: NP_795953.2
• NCBI GenBank Nucleotide #: NM_176979.5
• UniProt Accession #: Q6ZQF0; Q6P6P0; Q80Y33; Q8BUI0; Q8BUK1; Q8R348; Q91VX3; Q922X8
• Molecular Weight: 168,860 Da
• NCBI Official Full Name: DNA topoisomerase 2-binding protein 1
• NCBI Official Synonym Full Names: topoisomerase (DNA) II binding protein 1
• NCBI Official Symbol: Topbp1
• NCBI Official Synonym Symbols: AI256758; mKIAA0259; 1110031N14Rik; 2810429C13Rik; D430026L04Rik
• NCBI Protein Information: DNA topoisomerase 2-binding protein 1
• UniProt Protein Name: DNA topoisomerase 2-binding protein 1
• Protein Family: DNA topoisomerase 2-binding protein
• UniProt Gene Name: Topbp1
• UniProt Synonym Gene Names: Kiaa0259; TopBP1
• UniProt Entry Name: TOPB1_MOUSE

Uniprot Description

TOPBP1: a DNA-binding protein and scaffolding protein that is required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. Contains 7 BRCT domains, which are characteristic of proteins involved in cell cycle checkpoint control in response to DNA damage. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase. Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Up-regulated during the S phase of the cell cycle. Up-regulated by E2F1 and interferon.

Protein type: DNA replication

Cellular Component: male germ cell nucleus; Golgi apparatus; centrosome; PML body; cytoskeleton; condensed nuclear chromosome; cytoplasm; chromosome; nucleus; actin cytoskeleton

Molecular Function: identical protein binding; DNA binding

Biological Process: meiotic recombination; response to ionizing radiation; DNA repair; response to DNA damage stimulus