TNFAIP3 sirna

TNFAIP3 siRNA (Mouse)

Cat. Num. AAA8217080

• Gene Names: Tnfaip3; A20; Tnfip3
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: TNFAIP3; TNFAIP3 siRNA (Mouse); TNFIP3; Tumor necrosis factor alpha-induced protein 3; TNF alpha-induced protein 3; Putative DNA-binding protein A20; Zinc finger protein A20; TNFAIP3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: TNFAIP3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 775

• Applicable Applications for TNFAIP3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse TNFAIP3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for TNFAIP3 sirna

siRNA to inhibit TNFAIP3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 261862307
• NCBI GeneID: 21929
• NCBI Accession #: NP_033423.3
• NCBI GenBank Nucleotide #: NM_009397.3
• UniProt Accession #: Q60769; Q3U968
• Molecular Weight: 87,654 Da
• NCBI Official Full Name: tumor necrosis factor alpha-induced protein 3 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor, alpha-induced protein 3
• NCBI Official Symbol: Tnfaip3
• NCBI Official Synonym Symbols: A20; Tnfip3
• NCBI Protein Information: tumor necrosis factor alpha-induced protein 3
• UniProt Protein Name: Tumor necrosis factor alpha-induced protein 3
• Protein Family: TNFAIP3-interacting protein
• UniProt Gene Name: Tnfaip3
• UniProt Synonym Gene Names: Tnfip3; TNF alpha-induced protein 3
• UniProt Entry Name: TNAP3_MOUSE

Uniprot Description

TNFAIP3: Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Homodimer. Interacts with TNIP1, TAX1BP1 and TRAF2. Interacts with RNF11, ITCH and TAX1BP1 only after TNF stimulation; these interaction are transient and they are lost after 1 hour of stimulation with TNF. Interacts with YWHAZ and YWHAH. Interacts with IKBKG; the interaction is induced by TNF stimulation and by polyubiquitin. Interacts with RIPK1. Interacts with UBE2N; the interaction requires TAX1BP1. Interacts with TRAF6; the interaction is inhibited by HTLV-1 protein Tax. By TNF. Belongs to the peptidase C64 family.

Protein type: Apoptosis; EC 3.4.19.12; Protease; Ubiquitin conjugating system

Cellular Component: centrosome; lysosome; cytoplasm; nucleus

Molecular Function: ubiquitin binding; protease binding; protein self-association; zinc ion binding; hydrolase activity; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; ubiquitin-specific protease activity; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; peptidase activity; protein binding; DNA binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; kinase binding; catalytic activity; cysteine-type peptidase activity; ligase activity

Biological Process: negative regulation of autophagy; negative regulation of toll-like receptor 5 signaling pathway; negative regulation of smooth muscle cell proliferation; apoptosis; metabolic process; regulation of immunoglobulin production; proteolysis; negative regulation of toll-like receptor 3 signaling pathway; negative regulation of interleukin-2 production; negative regulation of innate immune response; response to molecule of bacterial origin; response to wounding; negative regulation of interleukin-6 production; inflammatory response; negative regulation of chronic inflammatory response; protein deubiquitination; negative regulation of heterotypic cell-cell adhesion; B-1 B cell homeostasis; negative regulation of cyclin-dependent protein kinase activity; negative regulation of B cell activation; negative regulation of tumor necrosis factor production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interleukin-1 beta production; regulation of germinal center formation; regulation of innate immune response; marginal zone B cell differentiation; response to muramyl dipeptide; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; positive regulation of protein catabolic process; negative regulation of protein ubiquitination; negative regulation of granuloma formation