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Western Blot (WB) (GKAP1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the GKAP1 antibody detected the GKAP1 protein (arrow).)

Rabbit GKAP1 Polyclonal Antibody | anti-GKAP1 antibody

GKAP1 Antibody (C-term)

Gene Names
GKAP1; FKSG21; GKAP42
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
GKAP1; Polyclonal Antibody; GKAP1 Antibody (C-term); G kinase-anchoring protein 1; cGMP-dependent protein kinase-anchoring protein of 42 kDa; GKAP42; anti-GKAP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GKAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-359 amino acids from the C-terminal region of human GKAP1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
331-359
Sequence Length
315
Applicable Applications for anti-GKAP1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(GKAP1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the GKAP1 antibody detected the GKAP1 protein (arrow).)

Western Blot (WB) (GKAP1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the GKAP1 antibody detected the GKAP1 protein (arrow).)

Immunohistochemistry (IHC)

(GKAP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GKAP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (GKAP1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GKAP1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of GKAP1 (arrow) using rabbit polyclonal GKAP1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GKAP1 gene.)

Western Blot (WB) (Western blot analysis of GKAP1 (arrow) using rabbit polyclonal GKAP1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GKAP1 gene.)
Related Product Information for anti-GKAP1 antibody
GKAP1 is a protein that is highly similar to the
mouse cGMP-dependent protein kinase anchoring protein 42kDa. The
mouse protein has been found to localize with the Golgi and recruit
cGMP-dependent protein kinase I alpha to the Golgi in mouse testes.
It is thought to play a role in germ cell development. Transcript
variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-GKAP1 antibody
References
Lim, J., et al. Cell 125(4):801-814(2006)
Sweetser, D.A., et al. Genes Chromosomes Cancer 44(3):279-291(2005)
Yuasa, K., et al. J. Biol. Chem. 275(7):4897-4905(2000)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42078
NCBI Official Full Name
G kinase-anchoring protein 1 isoform b
NCBI Official Synonym Full Names
G kinase anchoring protein 1
NCBI Official Symbol
GKAP1
NCBI Official Synonym Symbols
FKSG21; GKAP42
NCBI Protein Information
G kinase-anchoring protein 1
UniProt Protein Name
G kinase-anchoring protein 1
UniProt Gene Name
GKAP1
UniProt Synonym Gene Names
GKAP42
UniProt Entry Name
GKAP1_HUMAN

NCBI Description

This gene encodes a protein that is highly similar to the mouse cGMP-dependent protein kinase anchoring protein 42kDa. The mouse protein has been found to localize with the Golgi and recruit cGMP-dependent protein kinase I alpha to the Golgi in mouse testes. It is thought to play a role in germ cell development. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

GKAP1: a protein that is highly similar to the mouse cGMP-dependent protein kinase anchoring protein 42kDa. The mouse protein has been found to localize with the Golgi and recruit cGMP-dependent protein kinase I alpha to the Golgi in mouse testes. It is thought to play a role in germ cell development. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q21.32

Cellular Component: Golgi apparatus

Biological Process: signal transduction

Research Articles on GKAP1

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Product Notes

The GKAP1 gkap1 (Catalog #AAA9203476) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 331-359. The GKAP1 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's GKAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the GKAP1 gkap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GKAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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