Human Estradiol receptor ELISA Kit | ER elisa kit

Human Estradiol receptor ELISA Kit

Cat. Num. AAA727923

• Gene Names: ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
• Reactivity: Human
• Synonyms: Estradiol receptor; Human Estradiol receptor ELISA Kit; ER elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This ER ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ER. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ER elisa kit

Principle of the Assay: ER ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ER antibody and an ER-HRP conjugate. The assay sample and buffer are incubated together with ER-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ER concentration since ER from samples and ER-HRP conjugate compete for the anti-ER antibody binding site. Since the number of sites is limited, as more sites are occupied by ER from the sample, fewer sites are left to bind ER-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ER concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ER elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 170295804
• NCBI GeneID: 2099
• NCBI Accession #: NP_001116214.1
• NCBI GenBank Nucleotide #: NM_001122742.1
• Molecular Weight: 35,547 Da
• NCBI Official Full Name: estrogen receptor isoform 1
• NCBI Official Synonym Full Names: estrogen receptor 1
• NCBI Official Symbol: ESR1
• NCBI Official Synonym Symbols: ER; ESR; Era; ESRA; ESTRR; NR3A1
• NCBI Protein Information: estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; nuclear receptor subfamily 3 group A member 1
• UniProt Protein Name: Estrogen receptor
• UniProt Gene Name: ESR1
• UniProt Synonym Gene Names: ESR; NR3A1; ER
• UniProt Entry Name: ESR1_HUMAN

NCBI Description

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014]

Uniprot Description

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; integral to membrane; plasma membrane; nucleus

Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity

Biological Process: estrogen receptor signaling pathway; positive regulation of nitric oxide biosynthetic process; uterus development; signal transduction; response to estradiol stimulus; regulation of apoptosis; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of transcription factor activity; positive regulation of nitric-oxide synthase activity; antral ovarian follicle growth; male gonad development; androgen metabolic process; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; steroid hormone mediated signaling; gene expression; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity

Disease: Breast Cancer; Migraine With Or Without Aura, Susceptibility To, 1; Myocardial Infarction, Susceptibility To; Estrogen Resistance