SYT8 sirna

SYT8 siRNA (Mouse)

Cat. Num. AAA8230717

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SYT8; SYT8 siRNA (Mouse); Synaptotagmin-8; Synaptotagmin VIII; SytVIII; SYT8 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SYT8 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 389

• Applicable Applications for SYT8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SYT8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SYT8 sirna

siRNA to inhibit SYT8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 552953698
• NCBI GeneID: 55925
• NCBI Accession #: NP_001272786.1
• NCBI GenBank Nucleotide #: NM_001285857.1
• UniProt Accession #: Q9R0N6; Q64366; Q8C6E7; A9JAL0
• Molecular Weight: 43,804 Da
• NCBI Official Full Name: synaptotagmin-8 isoform b
• NCBI Official Synonym Full Names: synaptotagmin VIII
• NCBI Official Symbol: Syt8
• NCBI Protein Information: synaptotagmin-8
• UniProt Protein Name: Synaptotagmin-8
• Protein Family: Synaptotagmin
• UniProt Gene Name: Syt8
• UniProt Synonym Gene Names: SytVIII
• UniProt Entry Name: SYT8_MOUSE

Uniprot Description

SYT8: Isoform 4 may play a role in the trafficking and exocytosis of secretory vesicles in non-neuronal tissues. Mediates Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. May mediate Ca(2+)-regulation of exocytosis in insulin secreted cells. Belongs to the synaptotagmin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Vesicle

Cellular Component: synaptic vesicle membrane; membrane; cytoplasm; plasma membrane; integral to membrane; cytoplasmic vesicle

Molecular Function: clathrin binding; syntaxin binding; calcium-dependent protein binding

Biological Process: vesicle fusion; synaptic vesicle exocytosis; synaptic vesicle endocytosis; calcium ion-dependent exocytosis of neurotransmitter; acrosome reaction