Western Blot (WB)
(Anti-Osteopontin antibody, MBS175355, Western blottingLane 1: MM231 Cell LysateLane 2: HELA Cell LysateLane 3: JURKAT Cell LysateLane 4: COLO320 Cell Lysate )
Rabbit anti-Human Osteopontin Polyclonal Antibody | anti-SPP1 antibody
Anti-Osteopontin antibody
Western Blot (WB)
(Anti-Osteopontin antibody, MBS175355, Western blottingLane 1: MM231 Cell LysateLane 2: HELA Cell LysateLane 3: JURKAT Cell LysateLane 4: COLO320 Cell Lysate )
Western Blot (WB)
(Anti-Osteopontin antibody, MBS175355, Western blottingLane 1: MM231 Cell LysateLane 2: HELA Cell LysateLane 3: JURKAT Cell LysateLane 4: COLO320 Cell Lysate )
Immunohistochemistry (IHC)
(Anti-Osteopontin antibody, MBS175355, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-Osteopontin antibody, MBS175355, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Background: Osteopontin(OPN) also called urinary stone protein, secreted phosphoprotein 1(SPP1), bone sialoprotein, and early T lymphocyte activation 1(ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4q22.1
Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; extracellular region
Molecular Function: protein binding; extracellular matrix binding; cytokine activity
Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; decidualization; osteoblast differentiation; extracellular matrix disassembly; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; inflammatory response; cell adhesion; embryo implantation
Research Articles on SPP1
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Product Notes
The SPP1 spp1 (Catalog #AAA175355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Osteopontin antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Osteopontin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the SPP1 spp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Osteopontin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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