SRSF2 sirna

SRSF2 siRNA (Mouse)

Cat. Num. AAA8241107

• Gene Names: Srsf2; SC35; MRF-1; Pr264; Sfrs2; Sfrs10; D11Wsu175e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SRSF2; SRSF2 siRNA (Mouse); PR264; SFRS10; SFRS2; Serine/arginine-rich splicing factor 2; Protein PR264; Putative myelin regulatory factor 1; MRF-1; Splicing component. 35 kDa; Splicing factor SC35; SC-35; Splicing factor. arginine/serine-rich 2; SRSF2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SRSF2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 221

• Applicable Applications for SRSF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SRSF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SRSF2 sirna

siRNA to inhibit SRSF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6755478
• NCBI GeneID: 20382
• NCBI Accession #: NP_035488.1
• NCBI GenBank Nucleotide #: NM_011358.2
• UniProt Accession #: Q62093; Q542L3; Q60701
• Molecular Weight: 25,476 Da
• NCBI Official Full Name: serine/arginine-rich splicing factor 2
• NCBI Official Synonym Full Names: serine/arginine-rich splicing factor 2
• NCBI Official Symbol: Srsf2
• NCBI Official Synonym Symbols: SC35; MRF-1; Pr264; Sfrs2; Sfrs10; D11Wsu175e
• NCBI Protein Information: serine/arginine-rich splicing factor 2
• UniProt Protein Name: Serine/arginine-rich splicing factor 2
• Protein Family: Serine/arginine-rich splicing factor
• UniProt Gene Name: Srsf2
• UniProt Synonym Gene Names: Pr264; Sfrs10; Sfrs2; MRF-1; SC-35
• UniProt Entry Name: SRSF2_MOUSE

NCBI Description

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. [provided by RefSeq, Sep 2010]

Uniprot Description

SFRS2: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre- mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. The phosphorylated form (by SRPK2) is required for cellular apoptosis in response to cisplatin treatment. Belongs to the splicing factor SR family.

Protein type: RNA processing; Spliceosome; RNA splicing

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; nucleic acid binding; RNA binding; nucleotide binding

Biological Process: RNA splicing; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome