SNAPIN sirna

SNAPIN siRNA (Mouse)

Cat. Num. AAA8224104

• Gene Names: Snapin; 25kDa; Snapap; Bloc1s7; AA407989; AV026596; Snap25bp
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SNAPIN; SNAPIN siRNA (Mouse); BLOC1S7; SNAP25BP; SNAPAP; SNARE-associated protein Snapin; Biogenesis of lysosome-related organelles complex 1 subunit 7; BLOC-1 subunit 7; Synaptosomal-associated protein 25-binding protein; SNAP-associated protein; SNAPIN sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SNAPIN siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 136

• Applicable Applications for SNAPIN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SNAPIN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SNAPIN sirna

siRNA to inhibit SNAPIN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19923056
• NCBI GeneID: 20615
• NCBI Accession #: NP_598615.1
• NCBI GenBank Nucleotide #: NM_133854.3
• UniProt Accession #: Q9Z266; Q3U8V4; Q922V7
• Molecular Weight: 9,451 Da
• NCBI Official Full Name: SNARE-associated protein Snapin
• NCBI Official Synonym Full Names: SNAP-associated protein
• NCBI Official Symbol: Snapin
• NCBI Official Synonym Symbols: 25kDa; Snapap; Bloc1s7; AA407989; AV026596; Snap25bp
• NCBI Protein Information: SNARE-associated protein Snapin
• UniProt Protein Name: SNARE-associated protein Snapin
• Protein Family: SNAPIN protein
• UniProt Gene Name: Snapin
• UniProt Synonym Gene Names: Bloc1s7; Snap25bp; Snapap; BLOC-1 subunit 7; SNAP-associated protein
• UniProt Entry Name: SNAPN_MOUSE

Uniprot Description

SNAPIN: a component of the SNARE complex of proteins that is required for synaptic vesicle docking and fusion. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells. Phosphorylation by PKA modulates its interaction with the SNARE complex.

Protein type: Vesicle

Cellular Component: Golgi apparatus; cell; cytosol; secretory granule; synaptic vesicle; membrane; perinuclear region of cytoplasm; cytoplasm; nucleolus; synapse; cytoplasmic vesicle; intracellular; cell junction

Molecular Function: SNARE binding; protein binding

Biological Process: synaptic vesicle fusion to presynaptic membrane; intracellular protein transport; synaptic transmission; synaptic vesicle exocytosis; exocytosis; synaptic vesicle maturation; regulation of protein binding; anterograde synaptic vesicle transport; synaptic vesicle transport; endosome to lysosome transport; anterograde axon cargo transport; neurite development; calcium ion-dependent exocytosis