SNAI2 sirna

SNAI2 siRNA (Mouse)

Cat. Num. AAA8214875

• Gene Names: Snai2; Slug; Slugh; Snail2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SNAI2; SNAI2 siRNA (Mouse); SLUG; SLUGH; Zinc finger protein SNAI2; Neural crest transcription factor Slug; Protein snail homolog 2; SNAI2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SNAI2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 269

• Applicable Applications for SNAI2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SNAI2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SNAI2 sirna

siRNA to inhibit SNAI2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6755576
• NCBI GeneID: 20583
• NCBI Accession #: NP_035545.1
• NCBI GenBank Nucleotide #: NM_011415.2
• UniProt Accession #: P97469; O09096; Q9R211
• Molecular Weight: 30,003 Da
• NCBI Official Full Name: zinc finger protein SNAI2
• NCBI Official Synonym Full Names: snail family zinc finger 2
• NCBI Official Symbol: Snai2
• NCBI Official Synonym Symbols: Slug; Slugh; Snail2
• NCBI Protein Information: zinc finger protein SNAI2
• UniProt Protein Name: Zinc finger protein SNAI2
• Protein Family: Zinc finger protein
• UniProt Gene Name: Snai2
• UniProt Synonym Gene Names: Slug; Slugh
• UniProt Entry Name: SNAI2_MOUSE

Uniprot Description

Snail2: Transcriptional repressor. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains). Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. Belongs to the snail C2H2-type zinc-finger protein family.

Protein type: Motility/polarity/chemotaxis; C2H2-type zinc finger protein; Transcription factor; Apoptosis

Cellular Component: nuclear chromatin; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; nucleic acid binding; DNA binding; sequence-specific DNA binding; metal ion binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; cell migration; substrate-bound cell migration, cell release from substrate; Notch signaling pathway; transcription, DNA-dependent; regulation of osteoblast differentiation; neural crest cell development; multicellular organismal development; negative regulation of chondrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; palate development; Wnt receptor signaling pathway through beta-catenin; regulation of apoptosis; regulation of transcription from RNA polymerase II promoter; negative regulation of DNA damage response, signal transduction by p53 class mediator; response to radiation; sensory perception of sound; pigmentation; regulation of transcription, DNA-dependent; white fat cell differentiation; positive regulation of fat cell differentiation; epithelial to mesenchymal transition; positive regulation of histone acetylation; regulation of chemokine production; negative regulation of apoptosis; positive regulation of cell migration

Disease: Piebald Trait