Typical Testing Data/Standard Curve (for reference only)
Guinea Pig Muscarinic acetylcholine receptor ELISA Kit | M-AChR elisa kit
Guinea pig Muscarinic acetylcholine receptor ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This M-AChRenzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forM-AChR. Standards or samples are then added to the microtiter plate wells and M-AChRif present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of M-AChRpresent in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for M-AChRare added to each well to "sandwich" the M-AChRimmobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain M-AChRand enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
NCBI and Uniprot Product Information
NCBI Description
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract. The gene encoding this receptor is localized to 11q13. [provided by RefSeq, Jul 2008]
Uniprot Description
mAChR m1: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.
Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: asymmetric synapse; postsynaptic membrane; membrane; integral to plasma membrane; postsynaptic density; dendrite; plasma membrane; nerve terminal; cell junction
Molecular Function: drug binding; phosphoinositide phospholipase C activity; G-protein coupled acetylcholine receptor activity
Biological Process: nervous system development; acetylcholine receptor signaling, muscarinic pathway; protein modification process; signal transduction; muscarinic acetylcholine receptor, phospholipase C activating pathway; regulation of locomotion; cell proliferation; G-protein coupled receptor protein signaling pathway; positive regulation of cell proliferation; protein kinase C activation; neuromuscular synaptic transmission; positive regulation of ion transport; cognition; saliva secretion
Research Articles on M-AChR
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Product Notes
The Guinea Pig M-AChR chrm1 (Catalog #AAA725831) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA725831 ELISA Kit recognizes Guinea Pig M-AChR. It is sometimes possible for the material contained within the vial of "Muscarinic acetylcholine receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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