SMARCA1 sirna

SMARCA1 siRNA (Mouse)

Cat. Num. AAA8223210

• Gene Names: Smarca1; Snf2l; 5730494M04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SMARCA1; SMARCA1 siRNA (Mouse); SNF2L; Probable global transcription activator SNF2L1; ATP-dependent helicase SMARCA1; DNA-dependent ATPase SNF2L; Nucleosome-remodeling factor subunit SNF2L; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 1; SMARCA1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SMARCA1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1062

• Applicable Applications for SMARCA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SMARCA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SMARCA1 sirna

siRNA to inhibit SMARCA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 595582135
• NCBI GeneID: 93761
• NCBI Accession #: NP_001277637.1
• NCBI GenBank Nucleotide #: NM_001290708.1
• UniProt Accession #: Q6PGB8; Q8BSS1; Q91Y58; B1AUP6; B1AUP7
• Molecular Weight: 123,465 Da
• NCBI Official Full Name: probable global transcription activator SNF2L1 isoform 2
• NCBI Official Synonym Full Names: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1
• NCBI Official Symbol: Smarca1
• NCBI Official Synonym Symbols: Snf2l; 5730494M04Rik
• NCBI Protein Information: probable global transcription activator SNF2L1
• UniProt Protein Name: Probable global transcription activator SNF2L1
• Protein Family: Probable global transcription activator
• UniProt Gene Name: Smarca1
• UniProt Synonym Gene Names: Snf2l
• UniProt Entry Name: SMCA1_MOUSE

Uniprot Description

SMARCA1: Energy-transducing component of NURF (nucleosome- remodeling factor) and CERF (CECR2-containing-remodeling factor) complexes. Both complexes facilitate the perturbation of chromatin structure in an ATP-dependent manner. Potentiates neurite outgrowth. May be involved in brain development by regulating En-1 and En-2 expression. May be involved in the development of luteal cells. Part of the nucleosome-remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Part of the CECR2-containing-remodeling factor (CERF) complex which contains CECR2 and SMARCA1. Interacts with PRLR. Belongs to the SNF2/RAD54 helicase family. ISWI subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation; EC 3.6.1.-; Helicase; EC 3.6.4.-; Cell development/differentiation

Cellular Component: nuclear chromatin; nucleus

Molecular Function: DNA-dependent ATPase activity; hydrolase activity, acting on acid anhydrides; DNA binding; nucleic acid binding; hydrolase activity; ATPase activity; nucleosome binding; nucleotide binding; chromatin binding; helicase activity; ATP binding; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides

Biological Process: chromatin remodeling; neuron differentiation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; DNA strand renaturation; ATP-dependent chromatin remodeling; chromatin modification; brain development