Human TERF1 Interacting Nuclear Factor 2 (TINF2) ELISA Kit | TINF2 elisa kit

Human TERF1 Interacting Nuclear Factor 2 (TINF2) ELISA Kit

Cat. Num. AAA455938

• Gene Names: TINF2; TIN2; DKCA3
• Reactivity: Human
• Synonyms: TERF1 Interacting Nuclear Factor 2 (TINF2); Human TERF1 Interacting Nuclear Factor 2 (TINF2) ELISA Kit; TIN2; TRF1-interacting nuclear protein 2; TINF2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TINF2. No significant cross-reactivity or interference between TINF2 and analogues was observed.
• Sequence Length: 137

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.068ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TINF2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TINF2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TINF2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TINF2 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TINF2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TINF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TINF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TINF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 151101266
• NCBI GeneID: 26277
• NCBI Accession #: NP_001092744.1
• NCBI GenBank Nucleotide #: NM_001099274.1
• UniProt Accession #: Q9BSI4; Q9H904; Q9UHC2; B3W5Q7
• Molecular Weight: 15,434 Da
• NCBI Official Full Name: TERF1-interacting nuclear factor 2 isoform 1
• NCBI Official Synonym Full Names: TERF1 interacting nuclear factor 2
• NCBI Official Symbol: TINF2
• NCBI Official Synonym Symbols: TIN2; DKCA3
• NCBI Protein Information: TERF1-interacting nuclear factor 2
• UniProt Protein Name: TERF1-interacting nuclear factor 2
• Protein Family: TERF1-interacting nuclear factor
• UniProt Gene Name: TINF2
• UniProt Synonym Gene Names: TIN2

NCBI Description

This gene encodes one of the proteins of the shelterin, or telosome, complex which protects telomeres by allowing the cell to distinguish between telomeres and regions of DNA damage. The protein encoded by this gene is a critical part of shelterin; it interacts with the three DNA-binding proteins of the shelterin complex, and it is important for assembly of the complex. Mutations in this gene cause dyskeratosis congenita (DKC), an inherited bone marrow failure syndrome. [provided by RefSeq, Mar 2010]

Uniprot Description

Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Plays a role in shelterin complex assembly. Isoform 1 may have additional role in tethering telomeres to the nuclear matrix.