SLC6A20A sirna

SLC6A20A siRNA (Mouse)

Cat. Num. AAA827186

• Gene Names: Slc6a20a; Xt3s1; Xtrp3s1; AU022428; A730081N20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC6A20A; SLC6A20A siRNA (Mouse); SLC6A20; XT3S1; XTM3S1; XTRP3S1; Sodium- and chloride-dependent transporter XTRP3A; IMINO-B; Solute carrier family 6 member 20A; X transporter protein 3 similar 1; SLC6A20A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SLC6A20A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 592

• Applicable Applications for SLC6A20A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SLC6A20A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC6A20A sirna

siRNA to inhibit SLC6A20A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 20982825
• NCBI GeneID: 102680
• NCBI Accession #: NP_631881.1
• NCBI GenBank Nucleotide #: NM_139142.2
• UniProt Accession #: Q8VDB9; Q3USE2
• Molecular Weight: 66,178 Da
• NCBI Official Full Name: sodium- and chloride-dependent transporter XTRP3A
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter), member 20A
• NCBI Official Symbol: Slc6a20a
• NCBI Official Synonym Symbols: Xt3s1; Xtrp3s1; AU022428; A730081N20Rik
• NCBI Protein Information: sodium- and chloride-dependent transporter XTRP3A
• UniProt Protein Name: Sodium- and chloride-dependent transporter XTRP3A
• Protein Family: Sodium- and chloride-dependent transporter
• UniProt Gene Name: Slc6a20a
• UniProt Entry Name: S620A_MOUSE

Uniprot Description

SLC6A20: Mediates the calcium-dependent uptake of imino acids such as L-proline, N-methyl-L-proline and pipecolate as well as N- methylated amino acids. Involved in the transport of glycine. Defects in SLC6A20 are a cause of hyperglycinuria (HG). It is a condition characterized by excess of glycine in the urine. In some cases it is associated with renal colic and renal oxalate stones. Defects in SLC6A20 are a cause of iminoglycinuria (IG). It is a disorder of renal tubular reabsorption of glycine and imino acids (proline and hydroxyproline), marked by excessive levels of all three substances in the urine. Haploinsufficiency of SLC6A20 combined with deficiency of the neutral amino acid transporter SLC6A19 or partially inactivating mutations in SLC36A2, is responsible for iminoglycinuria. Additional polymorphisms and mutations in SLC6A18 can contribute to the IG phenotype in some families. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A20 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral; Transporter

Cellular Component: membrane; integral to plasma membrane; apical plasma membrane; integral to membrane; plasma membrane

Molecular Function: symporter activity; neurotransmitter:sodium symporter activity; amino acid transmembrane transporter activity

Biological Process: glycine transport; transport; amino acid transport; neurotransmitter transport; proline transport