Western Blot (WB)
(Western blot analysis of ZN473 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). ZN473 (arrow) was detected using the purified Pab.)
Rabbit anti-Human ZN473 Polyclonal Antibody | anti-ZN473 antibody
ZN473, NT (ZNF473, KIAA1141, ZFP100, Zinc finger protein 473, Zinc finger protein 100 homolog) (APC)
Gene Names
ZNF473; ZN473; HZFP100
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ZN473; Polyclonal Antibody; NT (ZNF473; KIAA1141; ZFP100; Zinc finger protein 473; Zinc finger protein 100 homolog) (APC); ZNF473; Zinc finger protein 100 homolog; anti-ZN473 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-ZN473 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
ZN473 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185~215 amino acids from the N-terminal region of human ZN473.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of ZN473 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). ZN473 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of ZN473 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). ZN473 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(ZN473 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(ZN473 Antibody (N-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-ZN473 antibody
References
Wagner, E.J., et al. Mol. Cell. Biol. 26(17):6702-6712(2006). Wagner, E.J., et al. RNA 12(7):1206-1218(2006). Pillai, R.S., et al. Genes Dev. 17(18):2321-2333(2003). Dominski, Z., et al. Genes Dev. 16(1):58-71(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
100,182 Da
NCBI Official Full Name
zinc finger protein 473
NCBI Official Synonym Full Names
zinc finger protein 473
NCBI Official Symbol
ZNF473
NCBI Official Synonym Symbols
ZN473; HZFP100
NCBI Protein Information
zinc finger protein 473; zfp-100; zinc finger protein ZFP100; zinc finger protein 100 homolog
UniProt Protein Name
Zinc finger protein 473
UniProt Gene Name
ZNF473
UniProt Synonym Gene Names
KIAA1141; ZFP100; Zfp-100
UniProt Entry Name
ZN473_HUMAN
Uniprot Description
ZNF473: Involved in histone 3'-end pre-mRNA processing by associating with U7 snRNP and interacting with SLBP/pre-mRNA complex. Increases histone 3'-end pre-mRNA processing but has no effect on U7 snRNP levels, when overexpressed. Required for cell cycle progression from G1 to S phases. Belongs to the krueppel C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: nucleoplasm; Cajal body
Molecular Function: protein binding; DNA binding; metal ion binding
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; histone mRNA metabolic process; histone mRNA 3'-end processing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription
Research Articles on ZN473
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Product Notes
The ZN473 znf473 (Catalog #AAA6365525) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZN473, NT (ZNF473, KIAA1141, ZFP100, Zinc finger protein 473, Zinc finger protein 100 homolog) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZN473 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ZN473 znf473 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZN473, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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