SLC26A5 sirna

SLC26A5 siRNA (Mouse)

Cat. Num. AAA8203813

• Gene Names: Slc26a5; Pres; prestin
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SLC26A5; SLC26A5 siRNA (Mouse); PRES; Prestin; Solute carrier family 26 member 5; SLC26A5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SLC26A5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 707

• Applicable Applications for SLC26A5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SLC26A5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SLC26A5 sirna

siRNA to inhibit SLC26A5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 577019546
• NCBI GeneID: 80979
• NCBI Accession #: NP_001276716.1
• NCBI GenBank Nucleotide #: NM_001289787.1
• UniProt Accession #: Q99NH7; Q80ZB1
• Molecular Weight: 81,337 Da
• NCBI Official Full Name: prestin isoform 2
• NCBI Official Synonym Full Names: solute carrier family 26, member 5
• NCBI Official Symbol: Slc26a5
• NCBI Official Synonym Symbols: Pres; prestin
• NCBI Protein Information: prestin
• UniProt Protein Name: Prestin
• Protein Family: Prestin
• UniProt Gene Name: Slc26a5
• UniProt Synonym Gene Names: Pres
• UniProt Entry Name: S26A5_MOUSE

Uniprot Description

SLC26A5: Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage- to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site. Defects in SLC26A5 are the cause of deafness autosomal recessive type 61 (DFNB61). A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter; Transporter, SLC family; Membrane protein, integral

Cellular Component: membrane; basolateral plasma membrane; cytoplasm; plasma membrane; integral to membrane; lateral plasma membrane

Molecular Function: protein binding; protein homodimerization activity; spectrin binding; sulfate transmembrane transporter activity; secondary active sulfate transmembrane transporter activity; transcription factor binding

Biological Process: regulation of cell shape; regulation of membrane potential; sensory perception of sound; positive regulation of cell size; fructose transport; sulfate transport; transmembrane transport; protein tetramerization