SETD8 sirna

SETD8 siRNA (Mouse)

Cat. Num. AAA8201659

• Gene Names: Setd8; PR-SET7; AA617402; AW536475; PR/SET07; 2410195B05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SETD8; SETD8 siRNA (Mouse); N-lysine methyltransferase SETD8; H4-K20-HMTase SETD8; Histone-lysine N-methyltransferase SETD8; PR/SET domain-containing protein 07; PR-Set7; PR/SET07; SET domain-containing protein 8; SETD8 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SETD8 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 350

• Applicable Applications for SETD8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SETD8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SETD8 sirna

siRNA to inhibit SETD8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 45580702
• NCBI GeneID: 67956
• NCBI Accession #: NP_084517.2
• NCBI GenBank Nucleotide #: NM_030241.3
• UniProt Accession #: Q2YDW7; Q8C0J9
• Molecular Weight: 38,845 Da
• NCBI Official Full Name: N-lysine methyltransferase SETD8 isoform 1
• NCBI Official Synonym Full Names: SET domain containing (lysine methyltransferase) 8
• NCBI Official Symbol: Setd8
• NCBI Official Synonym Symbols: PR-SET7; AA617402; AW536475; PR/SET07; 2410195B05Rik
• NCBI Protein Information: N-lysine methyltransferase SETD8
• UniProt Protein Name: N-lysine methyltransferase SETD8
• UniProt Gene Name: Setd8
• UniProt Synonym Gene Names: PR-Set7; PR/SET07
• UniProt Entry Name: SETD8_MOUSE

Uniprot Description

SETD8: a protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Methylates K20 of histone H4 (H4K20me1), a specific tag for epigenetic transcriptional repression. SETD8 protein and H4K20me1 levels are cell cycle regulated, both increasing in S phase and peaking at G2/M phase. Interacts with the PCNA protein, associates with sites of active DNA synthesis and is required for DNA replication and genome stability during S phase. Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle. Nucleosomes are preferred as substrate compared to free histones. Methylates p53K382, leading to repression of the pro-apoptotic and checkpoint activation functions of p53. SET8 expression levels decrease in response to DNA damage, allowing p53 to activate checkpoints and/or apoptosis. Both the methylation of histone H4K20 and p53K382 appear to be important for the functions of SET8 in S phase. Belongs to the histone-lysine methyltransferase family. PR/SET subfamily. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase

Cellular Component: nucleolus; chromosome; nucleus

Molecular Function: methyltransferase activity; trimethylamine methyltransferase activity; monomethylamine methyltransferase activity; RNA methyltransferase activity; tRNA (cytosine)-methyltransferase activity; rRNA (cytosine-C5-967)-methyltransferase activity; rRNA (uridine) methyltransferase activity; N-methyltransferase activity; tRNA (guanine) methyltransferase activity; histone-lysine N-methyltransferase activity; rRNA (adenine-N6,N6-)-dimethyltransferase activity; selenocysteine methyltransferase activity; arginine N-methyltransferase activity; protein-arginine N-methyltransferase activity; methanol-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N2-)-methyltransferase activity; dimethylamine methyltransferase activity; protein methyltransferase activity; 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity; transferase activity; hydroxyneurosporene-O-methyltransferase activity; rRNA (uridine-2'-O-)-methyltransferase activity; tRNA (uracil) methyltransferase activity; rRNA (adenine) methyltransferase activity; C-methyltransferase activity; transcription corepressor activity; cobalt-precorrin-6B C5-methyltransferase activity; cobalt-precorrin-3 C17-methyltransferase activity; 1-phenanthrol methyltransferase activity; S-methyltransferase activity; lysine N-methyltransferase activity; DNA-methyltransferase activity; tRNA (cytosine-5-)-methyltransferase activity; tRNA (adenine)-methyltransferase activity; C-terminal protein carboxyl methyltransferase activity; rRNA (cytosine) methyltransferase activity; cobalt-precorrin-7 C15-methyltransferase activity; protein-arginine N5-methyltransferase activity; rRNA methyltransferase activity; p53 binding; protein-leucine O-methyltransferase activity; demethylmenaquinone methyltransferase activity; rRNA (adenine-N6-)-methyltransferase activity; tRNA methyltransferase activity; rRNA (guanine) methyltransferase activity; methylarsonite methyltransferase activity; mRNA methyltransferase activity; methylamine-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N1-)-methyltransferase activity; cobalt-precorrin-5B C1-methyltransferase activity; protein-lysine N-methyltransferase activity; O-methyltransferase activity; tRNA (adenine-N1-)-methyltransferase activity; tRNA (adenine-57, 58-N(1)-) methyltransferase activity

Biological Process: methylation; mitosis; transcription, DNA-dependent; regulation of transcription, DNA-dependent; cell division; peptidyl-lysine mono-methylation; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; cell cycle; regulation of DNA damage response, signal transduction by p53 class mediator