SEC24A sirna

SEC24A siRNA (Mouse)

Cat. Num. AAA8210628

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SEC24A; SEC24A siRNA (Mouse); Protein transport protein Sec24A; SEC24-related protein A; SEC24A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SEC24A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1089

• Applicable Applications for SEC24A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SEC24A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SEC24A sirna

siRNA to inhibit SEC24A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 595763308
• NCBI GeneID: 77371
• NCBI Accession #: NP_001277714.1
• NCBI GenBank Nucleotide #: NM_001290785.1
• UniProt Accession #: Q3U2P1; Q3TQ05; Q3TRG7; Q8BIS0; A2AA70; A2AA73; A4FUV4
• Molecular Weight: 63,411 Da
• NCBI Official Full Name: protein transport protein Sec24A isoform 2
• NCBI Official Synonym Full Names: Sec24 related gene family, member A (S. cerevisiae)
• NCBI Official Symbol: Sec24a
• NCBI Protein Information: protein transport protein Sec24A
• UniProt Protein Name: Protein transport protein Sec24A
• Protein Family: Protein transport protein
• UniProt Gene Name: Sec24a
• UniProt Entry Name: SC24A_MOUSE

Uniprot Description

SEC24A: Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex. COPII is composed of at least five proteins: the Sec23/24 complex, the Sec13/31 complex and Sar1. SEC24A is capable of forming heterodimers with SEC24B and SEC24C. Interacts with TMED2. Expressed in fibroblasts, hepatocytes, and lymphocytes. Belongs to the SEC23/SEC24 family. SEC24 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Cellular Component: Golgi apparatus; membrane; COPII vesicle coat; endoplasmic reticulum; cytoplasm

Molecular Function: zinc ion binding

Biological Process: vesicle-mediated transport; intracellular protein transport; regulation of low-density lipoprotein receptor biosynthetic process; protein transport; ER to Golgi vesicle-mediated transport; positive regulation of protein secretion; transport