SAT1 sirna

SAT1 siRNA (Mouse)

Cat. Num. AAA8209697

• Gene Names: Sat1; Sat; SSAT; AA617398
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SAT1; SAT1 siRNA (Mouse); SAT; Diamine acetyltransferase 1; Polyamine N-acetyltransferase 1; Putrescine acetyltransferase; Spermidine/spermine N(1)-acetyltransferase 1; SSAT; SSAT-1; SAT1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SAT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 96

• Applicable Applications for SAT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SAT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SAT1 sirna

siRNA to inhibit SAT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 630044866
• NCBI GeneID: 20229
• NCBI Accession #: NP_001278794.1
• NCBI GenBank Nucleotide #: NM_001291865.1
• UniProt Accession #: P48026; Q3U444
• Molecular Weight: 20,012 Da
• NCBI Official Full Name: diamine acetyltransferase 1 isoform 2
• NCBI Official Synonym Full Names: spermidine/spermine N1-acetyl transferase 1
• NCBI Official Symbol: Sat1
• NCBI Official Synonym Symbols: Sat; SSAT; AA617398
• NCBI Protein Information: diamine acetyltransferase 1
• UniProt Protein Name: Diamine acetyltransferase 1
• Protein Family: Serine acetyltransferase
• UniProt Gene Name: Sat1
• UniProt Synonym Gene Names: Sat; SSAT; SSAT-1
• UniProt Entry Name: SAT1_MOUSE

Uniprot Description

SAT: Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine. Defects in SAT1 may be a cause of keratosis follicularis spinulosa decalvans X-linked (KFSDX). A rare disorder affecting the skin and the eye. Affected men show thickening of the skin of the neck, ears, and extremities, especially the palms and soles, loss of eyebrows, eyelashes and beard, thickening of the eyelids with blepharitis and ectropion, and corneal degeneration. Belongs to the acetyltransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 2.3.1.57; Acetyltransferase

Cellular Component: cytoplasm; intracellular; cytosol

Molecular Function: transferase activity; N-acetyltransferase activity; transferase activity, transferring acyl groups; diamine N-acetyltransferase activity; spermidine binding

Biological Process: spermidine acetylation; spermine catabolic process; polyamine catabolic process; polyamine metabolic process; regulation of cell proliferation