Rabbit ERdj3 Polyclonal Antibody | anti-ERdj3 antibody

ERdj3 (K66) Polyclonal Antibody

Cat. Num. AAA3003430

• Gene Names: DNAJB11; DJ9; EDJ; Dj-9; ERj3; PKD6; ABBP2; ERdj3; ERj3p; ABBP-2; UNQ537; PRO1080
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ERdj3; Polyclonal Antibody; ERdj3 (K66) Polyclonal Antibody; DnaJ homolog subfamily B member 11; APOBEC1-binding protein 2; ABBP-2; ABBP2; DnaJ protein homolog 9; ER-associated DNAJ; ER-associated Hsp40 co-chaperone; Endoplasmic reticulum DNA J domain-containing protein 3; ER-resident protein ERdj3; ERj3p; HEDJ; Human DnaJ protein 9; hDj-9; PWP1-interacting protein 4; DNAJB11; EDJ; ERJ3; HDJ9; PSEC0121; UNQ537/PRO1080; anti-ERdj3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ERdj3 (K66) polyclonal antibody detects endogenous levels of ERdj3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 358

• Applicable Applications for anti-ERdj3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200; IP: 1:10-1:100
• Immunogen: Synthetic peptide, corresponding to amino acids 32-88 of Human ERdj3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ERdj3 (K66) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:PANC1 whole cell lysate (40ug) Lane3:The Lung tissue lysate of Mouse (40ug) Lane4:The Kidney tissue lysate of Rat (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of ERdj3 (K66) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)

Immunoprecipitation (IP)

(Immunoprecipitation of L02 whole cell lysate using ERdj3 (K66) pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 and lane 5 ). Lane 1 is 20% input. The Western Blot was probed using ERdj3 (K66) pAb # MBS3003430.“up-arrow” (supernatant) ; “down-arrow” (deposition))

Related Product Information for anti-ERdj3 antibody

Members of the heat shock protein 40 (HSP 40) family of proteins all contain a highly conserved J domain that associates with HSP 70 and regulates the function of HSP 70 by activating its adenosine triphosphatase activity. ERdj3, an HSP 40 chaperone, is expressed in the ER lumen, where it interacts with BiP, a molecule involved in retrotranslocating proteins out of the ER. ERdj3 also associates with several other protein substrates, including unfolded light chains, a nonsecreted Ig light chain mutant and a VSV-G ts045 mutant. Shiga toxin (Stx) is a bacterial tool that enzymatically inactivates the 28S rRNA, inhibiting protein synthesis of infected cells. Stx also interacts with ERdj3 and Sec 61 to form a complex through which proteins are retrotranslocated to the cytoplasm. ERdj3 may play a role in the ER quality control system.

NCBI and Uniprot Product Information

• NCBI GI #: 7706495
• NCBI GeneID: 51726
• NCBI Accession #: NP_057390.1
• NCBI GenBank Nucleotide #: NP_057390.1
• UniProt Accession #: Q9UBS4
• Molecular Weight: ~ 40kDa
• NCBI Official Full Name: dnaJ homolog subfamily B member 11 isoform 1
• NCBI Official Synonym Full Names: DnaJ heat shock protein family (Hsp40) member B11
• NCBI Official Symbol: DNAJB11
• NCBI Official Synonym Symbols: DJ9; EDJ; Dj-9; ERj3; PKD6; ABBP2; ERdj3; ERj3p; ABBP-2; UNQ537; PRO1080
• NCBI Protein Information: dnaJ homolog subfamily B member 11
• UniProt Protein Name: DnaJ homolog subfamily B member 11
• UniProt Gene Name: DNAJB11
• UniProt Synonym Gene Names: EDJ; ERJ3; HDJ9; ABBP-2; ERdj3; ERj3p; hDj-9
• UniProt Entry Name: DJB11_HUMAN

NCBI Description

This gene encodes a soluble glycoprotein of the endoplasmic reticulum (ER) lumen that functions as a co-chaperone of binding immunoglobulin protein, a 70 kilodalton heat shock protein chaperone required for the proper folding and assembly of proteins in the ER. The encoded protein contains a highly conserved J domain of about 70 amino acids with a characteristic His-Pro-Asp (HPD) motif and may regulate the activity of binding immunoglobulin protein by stimulating ATPase activity. [provided by RefSeq, Mar 2014]

Uniprot Description

DNAJB11: Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity.

Protein type: Secreted; RNA processing; Secreted, signal peptide; Chaperone; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 3q27.3

Cellular Component: membrane; endoplasmic reticulum lumen; endoplasmic reticulum; nucleus

Molecular Function: protein binding; unfolded protein binding

Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; protein folding; unfolded protein response; mRNA modification