RRM2B sirna

RRM2B siRNA (Mouse)

Cat. Num. AAA8208903

• Gene Names: Rrm2b; p53R2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RRM2B; RRM2B siRNA (Mouse); P53R2; Ribonucleoside-diphosphate reductase subunit M2 B; TP53-inducible ribonucleotide reductase M2 B; p53-inducible ribonucleotide reductase small subunit 2-like protein; p53R2; RRM2B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RRM2B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 351

• Applicable Applications for RRM2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RRM2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RRM2B sirna

siRNA to inhibit RRM2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 41054952
• NCBI GeneID: 382985
• NCBI Accession #: NP_955770.1
• NCBI GenBank Nucleotide #: NM_199476.1
• UniProt Accession #: Q6PEE3
• Molecular Weight: 40,804 Da
• NCBI Official Full Name: ribonucleoside-diphosphate reductase subunit M2 B
• NCBI Official Synonym Full Names: ribonucleotide reductase M2 B (TP53 inducible)
• NCBI Official Symbol: Rrm2b
• NCBI Official Synonym Symbols: p53R2
• NCBI Protein Information: ribonucleoside-diphosphate reductase subunit M2 B
• UniProt Protein Name: Ribonucleoside-diphosphate reductase subunit M2 B
• Protein Family: Ribonucleoside-diphosphate reductase
• UniProt Gene Name: Rrm2b
• UniProt Synonym Gene Names: P53r2; p53R2
• UniProt Entry Name: RIR2B_MOUSE

Uniprot Description

RRM2B: Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8A (MTDPS8A). A disorder due to mitochondrial dysfunction characterized by various combinations of neonatal hypotonia, neurological deterioration, respiratory distress, lactic acidosis, and renal tubulopathy. Defects in RRM2B are the cause of mitochondrial DNA depletion syndrome type 8B (MTDPS8B). A disease due to mitochondrial dysfunction and characterized by ophthalmoplegia, ptosis, gastrointestinal dysmotility, cachexia, peripheral neuropathy. Defects in RRM2B are the cause of progressive external ophthalmoplegia with mitochondrial DNA deletions autosomal dominant type 5 (PEOA5). A disorder characterized by progressive weakness of ocular muscles and levator muscle of the upper eyelid. In a minority of cases, it is associated with skeletal myopathy, which predominantly involves axial or proximal muscles and which causes abnormal fatigability and even permanent muscle weakness. Ragged-red fibers and atrophy are found on muscle biopsy. A large proportion of chronic ophthalmoplegias are associated with other symptoms, leading to a multisystemic pattern of this disease. Additional symptoms are variable, and may include cataracts, hearing loss, sensory axonal neuropathy, ataxia, depression, hypogonadism, and parkinsonism. Belongs to the ribonucleoside diphosphate reductase small chain family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.17.4.1; Oxidoreductase; Nucleotide Metabolism - pyrimidine; Other Amino Acids Metabolism - glutathione; Nucleotide Metabolism - purine

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: metal ion binding; oxidoreductase activity; ribonucleoside-diphosphate reductase activity

Biological Process: deoxyribonucleoside diphosphate metabolic process; renal system process; deoxyribonucleotide biosynthetic process; response to oxidative stress; mitochondrial DNA replication; DNA repair; deoxyribonucleoside triphosphate metabolic process; DNA replication; kidney development; response to DNA damage stimulus