ROCK1 sirna

ROCK1 siRNA (Mouse)

Cat. Num. AAA8201304

• Gene Names: Rock1; Rock-I; 1110055K06Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ROCK1; ROCK1 siRNA (Mouse); Rho-associated protein kinase 1; Rho-associated. coiled-coil-containing protein kinase 1; Rho-associated. coiled-coil-containing protein kinase I; ROCK-I; p160 ROCK-1; p160ROCK; ROCK1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ROCK1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,353

• Applicable Applications for ROCK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ROCK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ROCK1 sirna

siRNA to inhibit ROCK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6677759
• NCBI GeneID: 19877
• NCBI Accession #: NP_033097.1
• NCBI GenBank Nucleotide #: NM_009071.2
• UniProt Accession #: P70335; Q8C3G4; Q8C7H0
• Molecular Weight: 158,042 Da
• NCBI Official Full Name: rho-associated protein kinase 1
• NCBI Official Synonym Full Names: Rho-associated coiled-coil containing protein kinase 1
• NCBI Official Symbol: Rock1
• NCBI Official Synonym Symbols: Rock-I; 1110055K06Rik
• NCBI Protein Information: rho-associated protein kinase 1
• UniProt Protein Name: Rho-associated protein kinase 1
• Protein Family: Rho-associated protein kinase
• UniProt Gene Name: Rock1
• UniProt Synonym Gene Names: ROCK-I; p160ROCK
• UniProt Entry Name: ROCK1_MOUSE

Uniprot Description

ROCK1: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Homodimer. Interacts with RHOB, RHOC, MYLC2B snd PTEN. Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Detected in blood platelets. Activated by RHOA binding. Inhibited by Y- 27632. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; DMPK family; ROCK subfamily

Cellular Component: ruffle; Golgi apparatus; cytoskeleton; cell projection; membrane; lamellipodium; cytoplasm; plasma membrane; intracellular

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; Rho GTPase binding; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; GTP-Rho binding; kinase activity; ATP binding; protein kinase activity

Biological Process: regulation of actin filament-based process; apoptosis; cytoskeleton organization and biogenesis; bleb formation; signal transduction; protein amino acid phosphorylation; Rho protein signal transduction; negative regulation of angiogenesis; leukocyte adhesion; positive regulation of focal adhesion formation; regulation of stress fiber formation; membrane to membrane docking; myoblast migration; negative regulation of neuron apoptosis; actin cytoskeleton organization and biogenesis; leukocyte tethering or rolling; regulation of keratinocyte differentiation; leukocyte migration; phosphorylation; regulation of cytoskeleton organization and biogenesis