Western Blot (WB)
(WB Suggested Anti-PPP2R4 AntibodyTitration: 1.0 ug/mlPositive Control: MDA-MB-435S Whole CellPPP2R4 is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB435 cells)
Rabbit anti-Human PTPA Polyclonal Antibody | anti-PTPA antibody
PTPA Antibody - N-terminal region
Western Blot (WB)
(WB Suggested Anti-PPP2R4 AntibodyTitration: 1.0 ug/mlPositive Control: MDA-MB-435S Whole CellPPP2R4 is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB435 cells)
Western Blot (WB)
(WB Suggested Anti-PPP2R4 AntibodyTitration: 1.0 ug/mlPositive Control: MDA-MB-435S Whole CellPPP2R4 is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB435 cells)
Target Description: Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.
NCBI and Uniprot Product Information
NCBI Description
Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
PPP2R4: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine- protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activty of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A. Associates with PP2A heterodimeric core enzyme PP2A(D), composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A). Interacts with PPP2CB. Widely expressed. Belongs to the PTPA-type PPIase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; EC 5.2.1.8; Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: nucleoplasm; protein phosphatase type 2A complex; cytoplasm; nucleus
Molecular Function: peptidyl-prolyl cis-trans isomerase activity; protein homodimerization activity; protein phosphatase 2A binding; protein heterodimerization activity; ATPase activity; protein phosphatase type 2A regulator activity; ATP binding; protein tyrosine phosphatase activator activity; receptor binding
Biological Process: positive regulation of phosphoprotein phosphatase activity; negative regulation of protein amino acid dephosphorylation; protein peptidyl-prolyl isomerization; positive regulation of apoptosis; metabolic process; mitotic spindle organization and biogenesis in nucleus; negative regulation of phosphoprotein phosphatase activity; regulation of phosphoprotein phosphatase activity; positive regulation of protein amino acid dephosphorylation
Research Articles on PTPA
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Product Notes
The PTPA ppp2r4 (Catalog #AAA3215461) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTPA Antibody - N-terminal region reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PTPA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the PTPA ppp2r4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: WKRSQAYADY IGFILTLNEG VKGKKLTFEY RVSEMWNEVH EEKEQAAKQS. It is sometimes possible for the material contained within the vial of "PTPA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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