RNF128 sirna

RNF128 siRNA (Mouse)

Cat. Num. AAA8233305

• Gene Names: Rnf128; GRAIL; Greul1; AI987883; 1300002C13Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RNF128; RNF128 siRNA (Mouse); GRAIL; GREUL1; E3 ubiquitin-protein ligase RNF128; Gene related to anergy in lymphocytes protein; Goliath-related E3 ubiquitin-protein ligase 1; RING finger protein 128; RNF128 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RNF128 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 402

• Applicable Applications for RNF128 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RNF128 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RNF128 sirna

siRNA to inhibit RNF128 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 363543360
• NCBI GeneID: 66889
• NCBI Accession #: NP_001241690.1
• NCBI GenBank Nucleotide #: NM_001254761.1
• UniProt Accession #: Q9D304; Q3UJY0; Q9CVG1; Q9DBN3; Q9JJF8
• Molecular Weight: 46,276 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF128 isoform 2
• NCBI Official Synonym Full Names: ring finger protein 128
• NCBI Official Symbol: Rnf128
• NCBI Official Synonym Symbols: GRAIL; Greul1; AI987883; 1300002C13Rik
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF128
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF128
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: Rnf128
• UniProt Synonym Gene Names: Grail; Greul1
• UniProt Entry Name: RN128_MOUSE

Uniprot Description

RNF128: E3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural- inducing signals. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin conjugating system; Membrane protein, integral; Ubiquitin ligase

Cellular Component: Golgi apparatus; cytoskeleton; membrane; endoplasmic reticulum; cytoplasm; late endosome; integral to membrane

Molecular Function: zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; metal ion binding; ribosomal S6-glutamic acid ligase activity; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: protein ubiquitination; negative regulation of cytokine biosynthetic process