RHOBTB3 sirna

RHOBTB3 siRNA (Mouse)

Cat. Num. AAA8204115

• Gene Names: Rhobtb3; AI317148; AW208826; mKIAA0878; 1700040C17Rik; 2610033K01Rik; 4930503C18Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RHOBTB3; RHOBTB3 siRNA (Mouse); KIAA0878; Rho-related BTB domain-containing protein 3; RHOBTB3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RHOBTB3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 611

• Applicable Applications for RHOBTB3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RHOBTB3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RHOBTB3 sirna

siRNA to inhibit RHOBTB3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31745135
• NCBI GeneID: 73296
• NCBI Accession #: NP_082769.1
• NCBI GenBank Nucleotide #: NM_028493.2
• UniProt Accession #: Q9CTN4; Q05DP2; Q3UTS4; Q80X55; Q9CVT0
• Molecular Weight: 69,208 Da
• NCBI Official Full Name: rho-related BTB domain-containing protein 3
• NCBI Official Synonym Full Names: Rho-related BTB domain containing 3
• NCBI Official Symbol: Rhobtb3
• NCBI Official Synonym Symbols: AI317148; AW208826; mKIAA0878; 1700040C17Rik; 2610033K01Rik; 4930503C18Rik
• NCBI Protein Information: rho-related BTB domain-containing protein 3
• UniProt Protein Name: Rho-related BTB domain-containing protein 3
• Protein Family: Rho-related BTB domain-containing protein
• UniProt Gene Name: Rhobtb3
• UniProt Synonym Gene Names: Kiaa0878
• UniProt Entry Name: RHBT3_MOUSE

Uniprot Description

RHOBTB3: Rab9-regulated ATPase required for endosome to Golgi transport. Involved in transport vesicle docking at the Golgi complex, possibly by participating in release M6PRBP1/TIP47 from vesicles to permit their efficient docking and fusion at the Golgi. Specifically binds Rab9, but not other Rab proteins. Has low intrinsic ATPase activity due to autoinhibition, which is relieved by Rab9.

Protein type: EC 3.6.1.-; Hydrolase

Cellular Component: Golgi apparatus; cell; cytoplasm; intracellular

Molecular Function: dihydroneopterin monophosphate phosphatase activity; UDP-2,3-diacylglucosamine hydrolase activity; GTP binding; hydrolase activity; ATP-dependent 5'-3' DNA helicase activity; ATPase activity; nucleotide binding; dATP pyrophosphohydrolase activity; 8-oxo-7,8-dihydroguanine triphosphatase activity; bis(5'-nucleosyl)-tetraphosphatase activity; protein binding; pyrophosphatase activity; thiamin-pyrophosphatase activity; dihydroneopterin triphosphate pyrophosphohydrolase activity; Rab GTPase binding; ATP binding

Biological Process: transport; metabolic process; small GTPase mediated signal transduction; retrograde transport, endosome to Golgi