REPS2 sirna

REPS2 siRNA (Mouse)

Cat. Num. AAA8219826

• Gene Names: Reps2; POB1
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: REPS2; REPS2 siRNA (Mouse); POB1; RalBP1-associated Eps domain-containing protein 2; Partner of RalBP1; RalBP1-interacting protein 2; REPS2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: REPS2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 648

• Applicable Applications for REPS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse REPS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for REPS2 sirna

siRNA to inhibit REPS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 84490401
• NCBI GeneID: 194590
• NCBI Accession #: NP_839987.2
• NCBI GenBank Nucleotide #: NM_178256.4
• UniProt Accession #: Q80XA6
• Molecular Weight: 57,750 Da
• NCBI Official Full Name: ralBP1-associated Eps domain-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: RALBP1 associated Eps domain containing protein 2
• NCBI Official Symbol: Reps2
• NCBI Official Synonym Symbols: POB1
• NCBI Protein Information: ralBP1-associated Eps domain-containing protein 2
• UniProt Protein Name: RalBP1-associated Eps domain-containing protein 2
• Protein Family: RalBP1-associated Eps domain-containing protein
• UniProt Gene Name: Reps2
• UniProt Synonym Gene Names: Pob1
• UniProt Entry Name: REPS2_MOUSE

Uniprot Description

REPS2: an adaptor protein involved in growth factor signaling through its influence on the Ral, a small G protein that regulates endocytosis of EGF and insulin receptors. Interacts with DDEF1, paxillin, RALBP1 and GRB2. Binding to RALBP1 does not affect the Ral-binding activity of the latter. Forms a ternary complex with activated Ral and RALBP1. Binds epsin 1. Relatively highly expressed in androgen-dependent as compared to androgen-independent prostate cancer cell lines and xenografts. EGF receptor internalization and signalling is inhibited by increased expression of REPS2. EGF stimulates phosphorylation on Tyr-residues and induces complex formation with EGF receptor through an adapter protein such as GRB2. Two alternatively spliced isoforms have been described. Isoform 2 is down-regulated during progression of prostate cancer.

Protein type: G protein regulator, misc.; Adaptor/scaffold

Cellular Component: cytoplasm

Molecular Function: metal ion binding; calcium ion binding