RBBP8 sirna

RBBP8 siRNA (Mouse)

Cat. Num. AAA8240340

• Gene Names: Rbbp8; RIM; CtIP; SAE2; RBBP-8; 9930104E21Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RBBP8; RBBP8 siRNA (Mouse); CTIP; DNA endonuclease RBBP8; CtBP-interacting protein; CtIP; Retinoblastoma-binding protein 8; RBBP-8; Retinoblastoma-interacting protein and myosin-like; RIM; Sporulation in the absence of SPO11 protein 2 homolog; SAE2; RBBP8 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RBBP8 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 893

• Applicable Applications for RBBP8 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RBBP8 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RBBP8 sirna

siRNA to inhibit RBBP8 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124487005
• NCBI GeneID: 225182
• NCBI Accession #: NP_001074692.1
• NCBI GenBank Nucleotide #: NM_001081223.2
• UniProt Accession #: Q80YR6
• Molecular Weight: 100,831 Da
• NCBI Official Full Name: DNA endonuclease RBBP8
• NCBI Official Synonym Full Names: retinoblastoma binding protein 8
• NCBI Official Symbol: Rbbp8
• NCBI Official Synonym Symbols: RIM; CtIP; SAE2; RBBP-8; 9930104E21Rik
• NCBI Protein Information: DNA endonuclease RBBP8
• UniProt Protein Name: DNA endonuclease RBBP8
• Protein Family: RBBP8 N-terminal-like protein
• UniProt Gene Name: Rbbp8
• UniProt Synonym Gene Names: Ctip; CtIP; RBBP-8; RIM; SAE2
• UniProt Entry Name: COM1_MOUSE

Uniprot Description

CtIP: a transcription factor containing C2H2 zinc fingers. May modulate the functions ascribed to BRCA1 in transcriptional regulation, DNA repair, and/or cell cycle checkpoint control. Interacts with CTBP, with the C-terminal (BRCT) domains of BRCA1, and with the retinoblastoma protein. Abundantly expressed in brain and the immune system and associated with immune system malignancies. Ionizing radiation induces its hyperphosphorylation and dissociation from BRCA1 in an ATM-dependent manner.

Protein type: EC 3.1.-.-; Transcription, coactivator/corepressor

Cellular Component: nucleoplasm; transcriptional repressor complex; nucleolus; nucleus

Molecular Function: nuclease activity; DNA binding; hydrolase activity; single-stranded DNA specific endodeoxyribonuclease activity; endonuclease activity; damaged DNA binding; T/G mismatch-specific endonuclease activity; phosphoric ester hydrolase activity

Biological Process: transcription from RNA polymerase II promoter; mitosis; negative regulation of transcription from RNA polymerase II promoter; cell cycle; DNA repair; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination; meiotic cell cycle; cell division; DNA damage checkpoint; blastocyst hatching; G2/M transition DNA damage checkpoint; response to DNA damage stimulus; G1/S transition of mitotic cell cycle