RAX sirna

RAX siRNA (Mouse)

Cat. Num. AAA8236029

• Gene Names: Rax; Rx; ey1; ey-1; 7530406A22Rik; E130303K03Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAX; RAX siRNA (Mouse); RX; Retinal homeobox protein Rx; Retina and anterior neural fold homeobox protein; RAX sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RAX siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 342

• Applicable Applications for RAX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RAX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAX sirna

siRNA to inhibit RAX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 89274179
• NCBI GeneID: 19434
• NCBI Accession #: NP_038861.2
• NCBI GenBank Nucleotide #: NM_013833.2
• UniProt Accession #: O35602; O08748; Q6GT92
• Molecular Weight: 36,335 Da
• NCBI Official Full Name: retinal homeobox protein Rx
• NCBI Official Synonym Full Names: retina and anterior neural fold homeobox
• NCBI Official Symbol: Rax
• NCBI Official Synonym Symbols: Rx; ey1; ey-1; 7530406A22Rik; E130303K03Rik
• NCBI Protein Information: retinal homeobox protein Rx
• UniProt Protein Name: Retinal homeobox protein Rx
• Protein Family: Retinal homeobox protein
• UniProt Gene Name: Rax
• UniProt Synonym Gene Names: Rx
• UniProt Entry Name: RX_MOUSE

Uniprot Description

RAX: Plays a critical role in eye formation by regulating the initial specification of retinal cells and/or their subsequent proliferation. Binds to the photoreceptor conserved element-I (PCE-1/Ret 1) in the photoreceptor cell-specific arrestin promoter. Defects in RAX are the cause of microphthalmia isolated type 3 (MCOP3). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present. Belongs to the paired homeobox family. Bicoid subfamily.

Protein type: DNA-binding

Cellular Component: nucleus

Molecular Function: DNA binding; sequence-specific DNA binding

Biological Process: transcription from RNA polymerase II promoter; limb development; hypothalamus development; camera-type eye development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; brain development; pattern specification process