RALGPS2 sirna

RALGPS2 siRNA (Mouse)

Cat. Num. AAA827185

• Gene Names: Ralgps2; AU043409; AW046161; 1810020P17Rik; 2210408F11Rik; 4921528G01Rik; 9130014M22Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RALGPS2; RALGPS2 siRNA (Mouse); Ras-specific guanine nucleotide-releasing factor RalGPS2; Ral GEF with PH domain and SH3-binding motif 2; RalA exchange factor RalGPS2; RALGPS2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RALGPS2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 590

• Applicable Applications for RALGPS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RALGPS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RALGPS2 sirna

siRNA to inhibit RALGPS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 229577168
• NCBI GeneID: 78255
• NCBI Accession #: NP_001153437.1
• NCBI GenBank Nucleotide #: NM_001159965.1
• UniProt Accession #: Q9ERD6; Q7TPZ6; Q80YA6; Q8BZ37; Q8BZU2; Q9D2Y7
• Molecular Weight: 62,783 Da
• NCBI Official Full Name: ras-specific guanine nucleotide-releasing factor RalGPS2 isoform a
• NCBI Official Synonym Full Names: Ral GEF with PH domain and SH3 binding motif 2
• NCBI Official Symbol: Ralgps2
• NCBI Official Synonym Symbols: AU043409; AW046161; 1810020P17Rik; 2210408F11Rik; 4921528G01Rik; 9130014M22Rik
• NCBI Protein Information: ras-specific guanine nucleotide-releasing factor RalGPS2
• UniProt Protein Name: Ras-specific guanine nucleotide-releasing factor RalGPS2
• Protein Family: Ras-specific guanine nucleotide-releasing factor
• UniProt Gene Name: Ralgps2
• UniProt Entry Name: RGPS2_MOUSE

Uniprot Description

RALGPS2: Guanine nucleotide exchange factor for the small GTPase RALA. May be involved in cytoskeletal organization. May also be involved in the stimulation of transcription in a Ras-independent fashion. RALGPS2 is a potential candidate gene for susceptibility to Alzheimer disease linked to 1q24. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Ras; GEFs

Cellular Component: membrane; cytoplasm; plasma membrane

Molecular Function: guanyl-nucleotide exchange factor activity

Biological Process: small GTPase mediated signal transduction