POLR1D sirna

POLR1D siRNA (Mouse)

Cat. Num. AAA8226653

• Gene Names: Polr1d; AC19; RPA16; Rpo1-3; 1110003G10Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: POLR1D; POLR1D siRNA (Mouse); RPO1-3; DNA-directed RNA polymerases I and III subunit RPAC2; RNA polymerases I and III subunit AC2; AC19; DNA-directed RNA polymerase I subunit D; RNA polymerase I 16 kDa subunit; RPA16; POLR1D sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: POLR1D siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 133

• Applicable Applications for POLR1D sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse POLR1D gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for POLR1D sirna

siRNA to inhibit POLR1D expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6677791
• NCBI GeneID: 20018
• NCBI Accession #: NP_033113.1
• NCBI GenBank Nucleotide #: NM_009087.2
• UniProt Accession #: P97304
• Molecular Weight: 15,072 Da
• NCBI Official Full Name: DNA-directed RNA polymerases I and III subunit RPAC2 isoform 1
• NCBI Official Synonym Full Names: polymerase (RNA) I polypeptide D
• NCBI Official Symbol: Polr1d
• NCBI Official Synonym Symbols: AC19; RPA16; Rpo1-3; 1110003G10Rik
• NCBI Protein Information: DNA-directed RNA polymerases I and III subunit RPAC2
• UniProt Protein Name: DNA-directed RNA polymerases I and III subunit RPAC2
• Protein Family: DNA-directed RNA polymerases
• UniProt Gene Name: Polr1d
• UniProt Synonym Gene Names: Rpo1-3; RNA polymerases I and III subunit AC2; RPA16
• UniProt Entry Name: RPAC2_MOUSE

NCBI Description

This gene encodes an RNA polymerase subunit that is a component of both the RNA polymerase I and RNA polymerase III complexes. RNA polymerase I is associated with transcription of pre-ribosomal RNAs, while RNA polymerase III is associated with transcription of small RNAs. Pseudogenes of this gene have been defined on chromosomes 4 and 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2013]

Uniprot Description

POLR1D: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common core component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. Defects in POLR1D are the cause of Treacher Collins syndrome type 2 (TCS2). A form of Treacher Collins syndrome, a disorder of craniofacial development. Treacher Collins syndrome is characterized by a combination of bilateral downward slanting of the palpebral fissures, colobomas of the lower eyelids with a paucity of eyelashes medial to the defect, hypoplasia of the facial bones, cleft palate, malformation of the external ears, atresia of the external auditory canals, and bilateral conductive hearing loss. Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - pyrimidine; Nucleotide Metabolism - purine

Cellular Component: DNA-directed RNA polymerase III complex; nucleus; DNA-directed RNA polymerase I complex

Molecular Function: protein dimerization activity; protein binding; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription, DNA-dependent