Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sodium/hydrogen exchanger 1 (SLC9A1) Standard Curve.)
Human Sodium/Hydrogen Exchanger 1 (SLC9A1) ELISA Kit | SLC9A1 elisa kit
Human Sodium/Hydrogen Exchanger 1 (SLC9A1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sodium/hydrogen exchanger 1 (SLC9A1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sodium/hydrogen exchanger 1 (SLC9A1) Standard Curve.)
Background: The antiporter is a ubiquitous membrane-bound enzyme involved in volume- and pH-regulation of vertebrate cells. It is inhibited by the non-specific diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others.The gene was first disrupted in mouse fibroblasts. The lost function was then restored by transfection with human genomic DNA. Clones containing these specific human sequences were isolated. One genomic fragment was identified as an exon-coding sequence from the sodium-hydrogen ion antiporter gene by demonstration that it could complement antiporter deficiency in mouse cells; that it recognized an mRNA in cells expressing antiport activity but not in deficient cells; and that it was amplified in variants overexpressing antiport activity.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011]
Uniprot Description
NHE1: sodium/hydrogen exchanger 1. Belongs to the Na(+)/H(+) exchanger family. Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient.
Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Motility/polarity/chemotaxis; Transporter
Chromosomal Location of Human Ortholog: 1p36.1-p35
Cellular Component: endoplasmic reticulum membrane; focal adhesion; cell surface; mitochondrion; basolateral plasma membrane; endoplasmic reticulum; integral to plasma membrane; T-tubule; integral to membrane; lipid raft; nucleoplasm; perinuclear region of cytoplasm; lamellipodium; apical plasma membrane; cytoplasm; plasma membrane
Molecular Function: protein binding, bridging; calmodulin binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein complex scaffold; solute:hydrogen antiporter activity; calcium-dependent protein binding; sodium:hydrogen antiporter activity; protein phosphatase 2B binding
Biological Process: positive regulation of NFAT protein import into nucleus; glycosaminoglycan metabolic process; pathogenesis; cardiac muscle cell differentiation; hyaluronan catabolic process; regulation of pH; positive regulation of action potential; cell growth; transmembrane transport; response to drug; cellular sodium ion homeostasis; cell migration; maintenance of cell polarity; positive regulation of cell growth; protein oligomerization; cellular response to insulin stimulus; regulation of stress fiber formation; carbohydrate metabolic process; regulation of focal adhesion formation; regulation of intracellular pH; ion transport; positive regulation of transcription from RNA polymerase II promoter; response to acidity; regulation of sensory perception of pain; hyaluronan metabolic process; negative regulation of apoptosis
Disease: Lichtenstein-knorr Syndrome
Research Articles on SLC9A1
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Product Notes
The Human SLC9A1 slc9a1 (Catalog #AAA9714249) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714249 ELISA Kit recognizes Human SLC9A1. It is sometimes possible for the material contained within the vial of "Sodium/Hydrogen Exchanger 1 (SLC9A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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