PDGFRB sirna

PDGFRB siRNA (Mouse)

Cat. Num. AAA8218397

• Gene Names: Pdgfrb; Pdgfr; CD140b; PDGFR-1; AI528809
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PDGFRB; PDGFRB siRNA (Mouse); PDGFR; PDGFR1; Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-derived growth factor receptor 1; PDGFR-1; CD antigen CD140b; PDGFRB sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: PDGFRB siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1099

• Applicable Applications for PDGFRB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse PDGFRB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PDGFRB sirna

siRNA to inhibit PDGFRB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226342982
• NCBI GeneID: 18596
• NCBI Accession #: NP_001139740.1
• NCBI GenBank Nucleotide #: NM_001146268.1
• UniProt Accession #: P05622
• Molecular Weight: 122,806 Da
• NCBI Official Full Name: platelet-derived growth factor receptor beta isoform 1
• NCBI Official Synonym Full Names: platelet derived growth factor receptor, beta polypeptide
• NCBI Official Symbol: Pdgfrb
• NCBI Official Synonym Symbols: Pdgfr; CD140b; PDGFR-1; AI528809
• NCBI Protein Information: platelet-derived growth factor receptor beta
• UniProt Protein Name: Platelet-derived growth factor receptor beta
• Protein Family: Platelet-derived growth factor receptor
• UniProt Gene Name: Pdgfrb
• UniProt Synonym Gene Names: Pdgfr; Pdgfr1; PDGF-R-beta; PDGFR-beta; PDGFR-1
• UniProt Entry Name: PGFRB_MOUSE

Uniprot Description

PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent.

Protein type: Kinase, protein; Membrane protein, integral; Oncoprotein; EC 2.7.10.1; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; PDGFR family

Cellular Component: focal adhesion; cell surface; membrane; lysosome; cytoplasm; apical plasma membrane; plasma membrane; integral to membrane; cytoplasmic vesicle; nucleus; intrinsic to plasma membrane

Molecular Function: platelet-derived growth factor binding; platelet-derived growth factor receptor binding; nucleotide binding; platelet-derived growth factor receptor activity; transmembrane receptor protein tyrosine kinase activity; protein kinase binding; protein kinase activity; transferase activity; signal transducer activity; protein binding; protein-tyrosine kinase activity; platelet-derived growth factor beta-receptor activity; transferase activity, transferring phosphorus-containing groups; phosphoinositide 3-kinase binding; kinase activity; ATP binding; receptor binding

Biological Process: positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; positive regulation of smooth muscle cell proliferation; multicellular organismal development; protein amino acid autophosphorylation; positive regulation of collagen biosynthetic process; cardiac myofibril assembly; platelet-derived growth factor receptor signaling pathway; chemotaxis; positive regulation of smooth muscle cell migration; signal transduction; protein amino acid phosphorylation; smooth muscle development; positive regulation of MAP kinase activity; nitrogen compound metabolic process; positive regulation of cell proliferation; tissue homeostasis; hemopoiesis; phosphatidylinositol metabolic process; kidney development; regulation of peptidyl-tyrosine phosphorylation; cell migration; phosphoinositide-mediated signaling; positive regulation of mitosis; positive regulation of chemotaxis; adrenal gland development; in utero embryonic development; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; glycosaminoglycan biosynthetic process; skeletal morphogenesis; regulation of actin cytoskeleton organization and biogenesis; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; positive regulation of cell migration